牙槽突裂牵张成骨胶原合成的研究

Collagen synthesis in distraction osteogenesis in alveolar cleft

目的:观察牙槽突裂牵张成骨整复过程中不同时期胶原合成的种类及演变过程,研究牵张新骨生成的方式及组织学特点。方法:在8只成年杂种犬建立牙槽突裂动物模型并行牵张成骨裂隙关闭术,在牵张结束0、14、28、63d分别处死实验动物取标本进行Ⅰ、Ⅱ、Ⅲ型胶原免疫组化染色,镜检分析。结果:牵张固定早期以分泌Ⅲ型胶原为主,随后Ⅰ型胶原增多并最终取代Ⅲ型胶原而成为骨基质的主要成分。Ⅱ型胶原在整个成骨过程中无明显阳性表现。结论:牙槽突牵张成骨以膜内成骨方式为主,无明显的软骨内成骨现象。

Objective: To observe collagen synthesis in the different phase of distraction osteogenesis(DO) in alveolar cleft.Methods:8 dogs were used to make experimental model of alveolar cleft and underwent the closure of the cleft by distraction osteogenesis.The animals were sacrificed on day 0, 14, 28 and 63 after completion of DO respectively. The samples were examined by histological observation and the expression of typeⅠ,Ⅱ,Ⅲ collagen was studied by immunohistochemistry staining. Results:On day 0, a lot of type Ⅲ collagen fibers were observed in the distraction area. On day 14, there was trabecula bone, collagen Ⅰexpression was stronger than collagen Ⅲ. On day 28, trabecula bone developed into network-like structure with type Ⅰ collagen expression. On day 63,lamellated bone with type Ⅰ collage expression filled the distraction area. No obvious expression of collagen Ⅱ was observed.Conclusions:The formation of new bone is mainly intramembrane osteogenesis along the distraction vector.