期刊文献+

17β-雌二醇对涎腺黏液表皮样癌高转移细胞M_3SP_4的作用

The effects of 17 beta-estradiol on the proliferation and metastasis of salivary mucoepidermoid carcinoma M_3SP_4 cells
下载PDF
导出
摘要 目的:研究17β雌二醇(E2)对涎腺黏液表皮样癌高转移细胞系M3SP4细胞的作用。方法:采用细胞记数法、MTT、软琼脂克隆形成法及相关癌基因免疫组织化学的方法研究17β雌二醇对M3SP4细胞的增殖和对VEGF、cerbB2、Ki67、P16的影响。结果:雌激素在10-10~10-5mol/L时对M3SP4有促进作用,其中在10-7mmol/L时对M3SP4的促增殖作用最显著,体外实验表明细胞群体倍增时间减少了10.8%,克隆形成率增加了225%,免疫组化显示VEGF、cerbB2、Ki67的表达增强,而P16的表达明显降低。体内实验,实验组裸鼠在每天给予0.0052mg/d的E2时,肿瘤细胞M3SP4的生长增加65%,转移增加了609%。VEGF、Ki67和cerbB2有较强表达,明显强于对照组;相反抑癌基因P16的表达较弱。结论:雌激素可刺激涎腺黏液表皮样癌M3SP4细胞的增殖和转移能力。 Objective:To study the effects of 17 beta-estradiol (E2) on the proliferation and metastasis potential of salivary gland mucoepidermoid carcinoma M_3SP_4 cells. Methods:The effects of E2 on the potential of proliferation and metastasis of M_3SP_4 cells were investigated by cell counting, MTT assay, clonegenetic assay, in vivo tumour growth and metastasis assay in nude mice.The expression of VEGF,c-erbB-2,Ki-67 and P16 in M_3SP_4 cells was examined by immunohistochemistry assay. Results:E2 increased the proliferation of M_3SP_4 cells at 10^-10-10^-5 mol/L. E2 at 10^-7 mol/L showed the strongest effects.After treatment with E2 at 10^-7 mol/L,the population doubling time of M_3SP_4 cells decreased by 10.8%,clonegenecity increased by 225%, VEGF,c-erbB-2 and Ki-67 expression increased,P16 decreased.In the in vivo assay,the tumour growth increased by 65% and metastasis increased by 609% in nude mice treated with E2 at 0.005 2 mg/d. Conclusion:E2 may stimulate the proliferation and metastasis potential of M_3SP_4 cells.
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2005年第4期499-503,共5页 Journal of Practical Stomatology
基金 国家自然科学基金资助编号:30371551
关键词 涎腺 黏液表皮样癌 17Β-雌二醇 Salivary gland Mucoepidermoid carcinoma 17beta-estradiol
  • 相关文献

参考文献4

二级参考文献16

  • 1关晓峰,邱蔚六,何荣根,林国础,周晓健.肺高转移性涎腺腺样囊性癌细胞株的筛选[J].中华口腔医学杂志,1996,31(2):74-77. 被引量:38
  • 2司徒镇强,细胞培养,1996年,173页
  • 3Jiang W G,J Surg,1994年,81卷,2期,1576页
  • 4左连富,流式细胞术样品制备技术,1991年,46页
  • 5Serrano M,Hannon GJ,Beach D,et al.A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4[J].Nature,1993,366(6456): 704-707.
  • 6Kamb A,Gruis NA,Weaver-Feldhaus J,et al.A cell cycle regulator potentially involved in genesis of many tumor types[J].Science,1994,264(5157): 436-440.
  • 7Nakayama H,Yasai W,Yokoaki H,et al.Reduced expression of nm23 associated with metastasis of human gastric carcinoma[J].Jpn J Cancer Res,1993,84(4):184-190.
  • 8Shintani S,Nakahara Y,Mihara M,et al.Inactivation of the p14(ARF),p15(INK4B) and p16(INK4A) genes is a frequent event in human oral squamous cell carcinomas[J].Oral Oncol,2001,37(6): 498-504.
  • 9Kim HS,Chung WB,Hong SH,et al.Inactivation of p16INK4a in primary tumors and cell lines of head and neck squamous cell carcinoma[J].Mol Cells,2000,10(5): 557-565.
  • 10Tsai CH,Yang CC,Chou LS,et al.The correlation between alteration of p16 gene and clinical status in oral squamous cell carcinoma[J].J Oral Pathol Med,2001,30(9): 527-531.

共引文献33

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部