头颈部放疗患者的口腔黏膜脱落细胞微核计数的初步研究

Micronucleus calculation in exfoliated cells of oral mucosa before and after radiotherapy in the patients with head and neck tumor

目的:探讨头颈部放疗患者的口腔黏膜脱落细胞微核数。方法:随访1978～2001年接受过头颈部放疗的患者30例、2002年接受放疗的头颈部肿瘤患者30例和健康志愿者30例,对口腔黏膜脱落细胞进行微核计数。结果:正常对照组、放疗前患者和放疗后患者三者间的脱落细胞微核数差异无统计学意义(P>0.05)。放疗结束时患者的脱落细胞微核数高于放疗前患者和放疗后患者的脱落细胞微核数,且有统计学意义(P<0.01)。结论:反映微核数的改变局限于肿瘤区和放射线对口腔黏膜上皮损伤的可逆转性,并提示脱落细胞微核数可用于放疗后患者肿瘤继发的监测。

Objective:To study the number of micronucleus in exfoliated cells of oral mucosa before and after radiotherapy in the patients with head and neck tumor. Methods:30 patients with head and neck tumor 1-22 years after radiation, 30 patients with head and neck tumor at completion of radiation of 40-86 Gy and 30 healthy controls were included. The micronucleus in exfoliated cells of oral mucosa were investigated by smear examination and Feulgen and fastgreen staining.Results:The micronuleus (% of cells with micronuleus) in the exfoliated cells of oral mucosa in the healthy controls was 0.18±0.14, in the patients before radiotherapy 0.18±0.12, in the patients at completion of radiotherapy 0.73±0.35, in the patients 1-22 years after radiotherapy 0.20±0.17.Conclusions:Radiotherapy may increase the number of micronucleus and the increace is reversible after radiotherapy.