甲基汞对雄性小鼠的生殖发育毒性及作用机制

Reproductive and developmental toxicities of male mice induced by methylmercury

目的:研究甲基汞对雄性小鼠的生殖发育毒性。方法:采用显性致死试验和精子畸变试验的方法研究不同剂量甲基汞对小鼠生殖发育的影响。结果:不同剂量的甲基汞可使雄性小鼠的交配率及雌鼠受孕率降低,其降低程度与染毒剂量有关,1/10LD50组受孕率是70.8%,但与阴性对照组比较差异无显著性(P>0.05)。剖检孕鼠所得子代小鼠的数量与亲代雄性小鼠的甲基汞染毒剂量有关,染毒各剂量组子代小鼠的窝平均活胎数、平均着床数明显减少,与阴性对照组比较差异具有显著性(P<0.05)。甲基汞可致雄性小鼠精子畸变率增加,除1/50LD50、1/10LD50组之间比较差异无显著性外,各剂量染毒组之间比较差异均具有显著性(P<0.01)。结论:甲基汞可使雄性小鼠的交配率和雌性小鼠的受孕率降低,窝平均活胎数和平均着床数减少,精子的畸变率增加,对雄性小鼠具有生殖毒性。

Objective To study the reproductive and developmental toxicities of methylmercury (MeHg) on male mice. Methods The dominant lethal test and the sperm abnormalities test were used to study the reproductive and developmental toxicities of MeHg on male mice. Results Different doses of MeHg could decrease the ratio of mating of male mice and the fertilization of female mice. The fertilization rate of 1/10 LD50 group was 70.8%, and it had no significant difference compared with the control group (P>0.05). Living fetus per litter, the mean nidation fetus and the amount of offspring were reduced by the increase of MeHg, and there were significant differences between experimental and control groups (P<0.05); It was observed that different MeHg exposure doses could also increase the sperm abnormalities rate and there were significant differences between all the experimental and control groups (P<0.01) except between 1/50 LD_50 and 1/10 LD_50 groups. Conclusion MeHg can cause the reproductive and developmental toxicities on male mice.