福辛普利对大鼠心肌缺血再灌注损伤的保护作用

Protective effects of Fosinopril on myocardial ischemia-reperfusion injury in rats

目的:观察福辛普利对大鼠心肌缺血再灌注损伤的保护作用。方法:采用在体大鼠结扎冠状动脉前降支30min后,松扎再灌注24h造成心肌缺血再灌注模型,测定血清天冬酸氨基转移酶(AST)、乳酸脱氢酶(LDH)、心肌型肌酸激酶同功酶(CKMB)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)活性及丙二醛(MDA),通过光镜和电镜观察心肌组织形态学。结果:福辛普利对心肌缺血30min再灌注损伤24h大鼠,可明显降低血清AST、LDH及CKMB活性(P<0.05),提高SOD及GSHPx活性,降低MDA(P<0.05或P<0.01),并能明显减轻心肌组织水肿以及超微结构的损伤。结论:福辛普利对大鼠心肌缺血再灌注损伤具有明显保护作用,可能与其增强抗氧化酶活性,减少自由基对心肌的氧化损伤有关。

Objective To observe the protective effects of Fosinopril on myocardial ischemia-reperfusion injury in rats. Methods The myocardial ischemia-reperfusion model was induced by 30 min coronary occulusion and 24 h reperfusion in opened-chest anesthetized rats. The changes of aspartate aminotransferase(AST), lactate dehydrogenase(LDH), MB isoenzyme of creatine kinase(CK-MB), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, and malondialdehyde (MDA) content in serum were determined. Morphology of myocardial tissue was observed with optics and electron microscopes. Results Fosinopril decreased significantly the serum MDA content and AST, LDH and CK-MB activities as well as increased the serum SOD and GSH-Px activities. It also reduced edema and injury of ultrastructure in myocardial tissue. Conclusion Fosinopril has protective effects on myocardial ischemia-reperfusion injury, which may be related to scavenging the oxygen free radicals formed during reperfusion.