哮喘儿童血清白细胞介素4、转化生长因子β_1及IgE水平的变化及临床意义

Changes of serum IL-4,TGFβ_1 and IgE levels in asthmatic children and their clinical significances

目的:探讨哮喘儿童血清白细胞介素4(IL4)、转化生长因子β1(TGFβ1)及IgE水平变化及临床意义。方法:对98例哮喘患儿应用双抗体夹心ELISA法,检测血清IL4、TGFβ1、IgE水平,其中发作期42例、缓解期56例;设正常对照52例。结果:在哮喘组,血清IL4、IgE水平明显高于对照组[(12.09±6.00)ng·L-1,(58.16±15.47)ng·L-1](P<0.05),TGFβ1水平显著低于对照组[(181.59±96.05)ng·L-1](P<0.05);在发作期血清IL4、IgE水平[(105.86±34.33)ng·L-1,(270.21±74.84)ng·L-1]均高于缓解期[(61.58±11.04)ng·L-1,(152.80±45.15)ng·L-1](P<0.05),在发作期血清TGFβ1水平[(41.97±12.77)ng·L-1]均低于缓解期[(63.40±23.40)ng·L-1](P<0.05)。IL4与IgE呈正相关(r=0.581,P<0.001),TGFβ1分别与IL4、IgE呈负相关(r1=-0.614,P<0.001;r2=-0.609,P<0.001)。结论:IL4可促进哮喘患儿IgE分泌,并抑制嗜酸性粒细胞产生TGFβ1,这一机理在哮喘发病中起重要作用。

Objective To explore the changes of serum IL-4, TGFβ1 and IgE levels in asthmatic children and their clinical significances. Methods The serum IL-4,TGFβ1 and IgE levels of ninety-eight asthmatic children, including forty-two in acute attack group and fifty-six in remission group, and fifty-two healthy children in normal control group were detected by Sandwich ELISA. Results The serum IL-4 and IgE levels in asthmatic groups increased (P<0.05),whereas TGFβ1 level decreased (P<0.04),Both IL-4 and IgE levels (ng·L^-1) in acute attack group (105.86±34.33,270.21±74.84) were higher than those in remission group (61.58±11.04,52.80±45.15)(P<0.05). But the TGFβ1 level (ng·L^-1) in acute group (41.97±12.77) was lower than that in remission group (63.40±23.40)(P<0.05). There was positive correlation between the levels of IL-4 and IgE (r=0.581,P<0.001). However, there were negative correlations between the levels of TGFβ1 and IL-4 or IgE (r1 =-0.614, P<0.001;r2=-0.609,P<0.001). Conclusion IL-4 can accelerate secretion of IgE in asthmatic children and restrain eosinophilic granulocyte from secreting TGFβ1, thereby play an important role in asthmatic pathogenesis.