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腺病毒介导的突变型p27对裸鼠大肠癌的抑制作用

Inhibitory effect of p27mt mediated by adenovirus on nude mice model of colorectal carcinoma
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摘要 目的:以腺病毒为载体,研究外源性突变型p27(p27mt)基因对大肠癌的体内抑制作用.方法:应用细胞移植法把人大肠癌SW480细胞接种于BALB/c裸鼠皮下,构建大肠癌裸鼠模型,将已成功构建的腺病毒介导的突变型p27(Adp27mt)及LacZ采用瘤体内直接注射法注射到瘤体内,与对照组比较,绘制肿瘤生长曲线,计算肿瘤生长抑制率,并进行细胞凋亡的相关检测.结果:Adp27mt基因治疗组肿瘤生长明显受到抑制,有明显的促凋亡作用,与LacZ、对照组比较,有显著性差异(P<0.01).结论:Adp27mt基因对大肠癌裸鼠模型的体内肿瘤有明显的抑制作用. AIM: To investigate the inhibitory effect of extrinsic p27mt gene mediated by adenovirus vector on colorectal carcinoma nude mice. METHODS: The model of colorectal carcinoma nude mice was established by inoculating the SW480 cells of human colorectal carcinoma. The Ad-p27mt and Lac-Z was injected into the tumors of treatment groups respectively. The growth curves and the growth inhibition rates of tumors in the treatment groups were analyzed and compared with those in the control group. RESULTS: The Ad-p27mt obviously suqpressed the growth of tumors and promoted apoptosis of SW480 cells, which were significantly different compared with those in the control group (P<0.01). CONCLUSION: The Ad-p27mt has obvious inhibitory effect on the tumor growth of colorectal carcinoma in nude mice in vivo.
作者 杜勇 徐少勇 陈珺 杨建业 王家宁 黄永章
机构地区 武汉大学医学院消化内科 郧阳医学院附属人民医院
出处 《第四军医大学学报》 北大核心 2005年第14期1272-1274,共3页 Journal of the Fourth Military Medical University
基金 湖北省自然科学基金(2003ABA193)
关键词 p27mt 结直肠肿瘤 疾病模型 动物 小鼠 裸 p27mt colorectal neoplasms disease models, animal mice, nude
分类号 R730.5 [医药卫生—肿瘤]
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参考文献7

  • 1Tokumoto YM, Apperly JA, Gao FB, et al. Possttranscrigenesis regulation of p18 and p27 Cdk inhibitor proteins and the timing of oligodendrocyte differentian [ J]. Dev Biol, 2002 ;245 ( 1 ) :224 - 234.
  • 2黎岳南,蒋洁,郑晖.p27和cyclin D_1蛋白在非霍奇金淋巴瘤中的表达及其意义[J].中国医师杂志,2003,5(10):1320-1322. 被引量：20
  • 3Wataru N, Tasusei K, Shuho S, et al. Reduced expression of cyclindependent kinase inhibitor p27Kip1 is associated with advanced stage and invasiveness of gastric carcinomas [ J ]. Jpn J Cancer Res,1997 ;88 (3) :625.
  • 4Shibata H, Matsubara O, Wakiyana H, et al. The role of cyclin-dependent kinase inhibitor p27 in squamous cell carcinoma of the esophagus [J]. Pathol Res Pract, 2001 ;197(3): 157 - 164.
  • 5Park KH, Seol JY, Kim TY, et al. An adenovirus expressing mutant p27 showed more potent antitumor effects than adenovirus-p27 wild type [J]. Cancer Res, 2001 ;61(16) :6163 -6169.
  • 6Lim MS, Adamson A, Lin Z, et al. Expression of Skp2, a p27 ( kip1 ) ubiquitin ligase , in malignant lymphoma : correlation with p27( kip1 ) and proliferation index [ J]. Blood, 2002; 100 ( 3 ):2950 - 2956.
  • 7Jean A, Hurteau MD, Bruthiewicz PHD, et al. Overexpression of a stabilized mutant form of the cyclin-dependent kinase inhibitor p27kip1 inhibits cell growth [ J]. Gynecol Oncol, 2002 ;86(10) :19-23.

二级参考文献11

  • 1Sanchez- Beato M, Camacho FI, Martinez- Montera JC, et al. Anomalous high p27/KIP1 expression in a subset of aggressive B - cell lymphomas is associated with cyclin D3 overexpression.p27/KIP1 - cyclin D3 colocalization in tumor cells[J]. Blood, 1999, 94(2) : 765 - 772.
  • 2Kudoh S, Kumaravel TS, Kuramavel B, et al. Protein expression of cell cycle regulator, p27Kipl, correlates with histopathological grade of non-Hodgkin's lymphoma[J]. Jpn J Cancer Res, 1999, 90(11):1262- 1269.
  • 3Isaacson PG, Norton AJ. Extranodal lymphomas[M]. Edinburgh: Churchill Livingstone, 1994,5 - 6.
  • 4Bresalier RS, Ho SB, Schoeppner, HL, et al. Enhanced sialylation of mucin- associated carbohydrate structures in human colon cancer metastasis[ J ].Gastroenterol, 1996, 110(5): 1354-1367.
  • 5Kim DH, Lee HI, Nam ES, et al. Reduced expression of the cell - cycle inhibitor p27Kip1 is associated with progression and lymph node melastasis of gastric carcinoma[J]. Histopathology, 2000, 36(3):245 -251.
  • 6Shin JY, Kim HS, Park J, et al. Mechanism for inactivation of the KIP family cyclin - dependent kinase inhibitor genes in gastric cancer cells[J]. Cancer Res, 2000, 60(2):262- 265.
  • 7Zhou P, Jiang W, Weghorst CM, et al. Overexpression of cyclin D1 enhences gene amplification[J]. Cancer Res, 1996,56:36-39.
  • 8Dreyling MFI, Bullinger L, Ott G, et al. Alterations of the cyelin D1/p16 -pRB pathway in mantle cell lymphoma[J]. Cancer Res, 1997,57:4608 -4614.
  • 9Sgambato A, Zhang YJ, Krber N, et al. Weinstein IB: Deregulated expression of p27kip1 in human Breast Cancers[J]. Clinical Cancer Res, 1997,3:1879 - 1887.
  • 10Doki Y, Imoto M,Han EK H,et al. Weinstein IB: Increased expression of the p27KIP1 protein in human esophageal cancer cell lines that over - express cyclin D1[J]. Carcinogenesis, 1997, 18:1139- 1148.

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第四军医大学学报
2005年 第14期

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