乙型肝炎病毒感染患儿肝内乙型肝炎X抗原的表达

EXPRESCION OF HBXAg IN THE LIVER OFCHILDREN WITH HEPATITIS B VIRUS IN-FECTION

应用抗ＨＢｘ单克隆抗体，以生物素。卵白素复合物法对４０例乙型肝炎病毒感染患儿肝内乙型肝炎Ｘ抗原（ＨＢｘＡｇ）的表达进行研究。结果表明，ＨＢｘＡｇ阳性率７０．７％，其中慢性活动性肝炎高达８８．９％；慢性活动性肝炎和肝硬化的阳性率明显高于急性肝炎和慢性迁延性肝炎（Ｐ＜０．０１）。ＨＢｃＡｇ阳性率７３．２％，各病理类型间无显著性差异；ＨＢｘＡｇ和ＨＢＣＨｇ的表达无明显一致性（Ｐ＞０．０５）。ＨＢｘＡｇ在肝细胞中呈胞浆型、浆膜型、膜型和核型，还表达于胆管细胞。提示ＨＢｘＡｇ肝内的表达与乙型肝炎病毒慢性感染有一定关系，但与病毒复制无明显一致性。

orty-one liver specimens from 40 children withhepatitis B virus(HBV) infection were studied for theexpression of HBxAg by the avidinbiotin-peroxidasecomplex technique using anti-HBxAg monoclonal anti-body. 70. 7% of specimens were HBxAg positive, witha positive rate up to 88. 9% in chronic active hepatitis.The positive rate of HBxAg in chronic active hepatitisand cirrhosis was significantly higher than that in acutehepatitis and chronic persistent hepatitis (P < 0. 01 ).73. 2 % of specimens were HBcAg positive and there wasno statistical difference in distinct pathological groups.The expressions of HBxAg and HBcAg in all specimensshowed no statistical correlation (P> 0. 05 ). HBxAgwas located in the cytoplasm and/or cell membrane and/or nuclei of hepatocytes, and also expressed in bile ductcells. These results suggest that HBxAg relates to thepersistence of chronic HBV infection rather than thereplication of HBV in hepatocytes.