摘要
目的:用荷瘤兔模型研究乏氧显像剂(4,9diaza3,3,10,10tetramethyldodecan2,11dionedioxime,99mTcHL91)和18F氟代脱氧葡萄糖(18Ffluorodeoxyglucose,18FFDG)在肿瘤乏氧诊断中的应用价值。方法:1)体外试验,分别观察在常氧及乏氧状态下对VX2细胞摄取99mTcHL91和18FFDG的影响。2)体内试验,分别于耳缘静脉注入99mTcHL91后3h和18FFDG后1h,采集荷瘤兔的图象,随后,处死荷瘤兔取脑、肺、心、肝、脾、肾、坏死和非坏死肿瘤等标本称质量,测定放射性计数。肿瘤组织切片行HE染色,观察其特点。结果:1)乏氧与常氧状态下比较能引起VX2细胞对99mTcHL91和18FFDG的摄取并随时间延长而增加,P<0.05。2)荷瘤兔肿瘤显像清晰,肿瘤未坏死部位的放射性分别是坏死部位的4倍(99mTcHL91)和3.5倍(18FFDG)。HE染色显示瘤内有大量的坏死组织。结论:乏氧能引起VX2细胞对99mTcHL91和18FFDG的摄取增加;VX2荷瘤兔的瘤体在99mTcHL91和18FFDG显像上均为清晰的阳性病灶,坏死肿瘤组织的放射性低于非坏死肿瘤组织;两者均不能将乏氧的部位明确地显示出来。
OBJECTIVE: To elucidate the application of 99m Tc-HL91 and 18 F-FDG in hypoxic diagnosis by the model of tumor bearing rabbit. METHODS: The uptake of 99m Tc-HL91 and 18 F-FDG under hypoxic and normoxic condition were compared respectively. In vivo, 99m Tc-HL91 and 18 F-FDG was injected intravenously, images were acquired 3h and 1h respectively, then the rabbits were killed. The specimen of muscle, brain, heart, liver, spleen, kidney, lung, necrotic tumor and non-necrotic tumor were weighted and radioactivity was counted by γ-counting well. The character of tumor was observed by HE stained. RESULTS: The uptakes of 99m Tc-HL91 and 18 F-FDG in hypoxic cells were higher than nomorxia cells. The uptakes increased with time prolonged, P<0.05. The tumors were found clearly in 99m Tc-HL91 SPECT and 18 F-FDG PET/CT images. But the radioactivity of venter was higher than tumor. The radioactivity of non-necrotic tumor was 4 times or 3.5 times of that of necrotic tumor using 99m Tc-HL91 or 18 F-FDG respectively. HE stained indicated that there was a lot of necrotic tissue in tumor. CONCLUSIONS: The uptake of 99m Tc-HL91 and 18 F-FDG were higher in hypoxic VX_2 cells than normoxic one; 99m Tc-HL91SPECT and 18 F-FDG PET/CT could identify regional tumor in rabbit bearing VX_2 cell. The radioactivity was higher in non-necrotic tumor than necrotic tumor; they could not identify hypoxic regional clearly.
出处
《肿瘤防治杂志》
2005年第12期881-884,共4页
China Journal of Cancer Prevention and Treatment
基金
国家自然基金资助(C030330302)
