保留式腹腔内温热化疗对小鼠肝癌腹水瘤的治疗作用

Therapeutic effects of retaining intraperitoneal hyperthermic and hypotonic chemotherapy in mice bearing murine hepatoma ascitogenous tumor cells

目的:观察保留式腹腔内温热化疗对小鼠肝癌腹水瘤的抑制作用,探讨其对卵巢癌的治疗。方法:建立小鼠肝癌腹水瘤模型,出现腹水后,腹腔内分别注入常温生理盐水+顺铂(常规腹腔化疗组)和温热、低渗液+顺铂(温热腹腔化疗组),比较两组腹水中的瘤细胞数和存活瘤细胞数,并观察荷瘤小鼠的存活时间和生命延长率。结果:1)腹腔常规化疗和温热低渗化疗组的瘤细胞计数[分别为(9.26±6.45)×106和(3.24±1.56)×106)]及存活瘤细胞数(分别为51.25%和34.69%)均较对照组[分别为(18.89±5.34)×106和95.76%]显著减少,而且腹腔温热化疗组明显低于常规化疗组。2)腹腔常规化疗组的小鼠荷瘤存活时间为(25.76±4.36)d,生命延长率为58.04%;腹腔内温热化疗组的荷瘤存活时间为(36.32±5.76)d,生命延长率为122.82%,两者差异有统计学意义。结论:腹腔内温热低渗化疗较常规化疗可明显抑制腹腔肿瘤的生长,延长荷瘤小鼠的存活时间,对临床治疗腹腔恶性肿瘤可能是一有效的方法。

OBJECTIVE:To evaluate the antitumoral efficacy of retaining intraperitoneal hyperthermic and hypotonic chemotherapy (IPHC) in mice bearing murine hepatoma ascitogenous H22 tumor cells.METHODS:The model of hepatoma ascitogenous tumor cells of KM mice was used.Room temperature normal saline (NS) with cisplatin and hypotonic solution (DDW) with cisplatin heated to 45℃ were injected intraperitoneally respectively after ascites were appeared.Tumor cell calculation,survival rate of tumor cells,survival time of the mice bearing tumor cells and the life-prolonging rate were investigated and compared separately.RESULTS:Compared with the control group tumor cells, the survival rate of the cells in KM mice peritoneal cavity significantly decreased in the two research groups, and the IPHC group was more lowered.Survival time and life-prolonging rate of the KM mice bearing tumor cells were (25.76±4.36) days and 58.04% in the room temperature chemotherapy group vs (36.32±5.76) days and 122.82% in the IPHC group.There was a significant difference between the two groups.CONCLUSIONS:Compared to the routine intraperitoneal chemotherapy IPHC can effectively inhibit the growth of tumor cells and prolong the suvival time of the mice bearing tumor cells.It may be an another effective method to treat malignant tumors occurred in peritoneal cavity.