卵巢上皮性癌组织中PTTG的表达与微血管密度和bFGF的关系

Relationship between microvessel density, basic fibroblast growth factor and PTTG expression in epithelial ovarian cancer

目的:探讨垂体肿瘤转化基因(PTTG)在卵巢上皮性癌组织中的表达及其与微血管密度(MVD)计数和碱性成纤维细胞生长因子(bFGF)蛋白表达的关系。方法:采用逆转录聚合酶链反应(RTPCR)技术,检测42例卵巢上皮性癌组织中PTTGmRNA的表达;应用免疫组织化学法,检测PTTG和bFGF蛋白的表达,CD34标记血管内皮细胞并计数MVD。以18例卵巢良性上皮性肿瘤和12例正常卵巢组织进行对照。结果:卵巢上皮性癌组织中PTTG高度表达,并与手术病理分期和淋巴结转移正相关,P值均<0.05;PTTG的表达与年龄、病理类型或组织学分级无关,P>0.05。卵巢上皮性癌组织中PTTG的表达与bFGF蛋白表达及微血管密度正相关,P值均<0.05。结论:卵巢上皮性癌组织中PTTG高度表达参与了卵巢上皮性癌的发生及演进过程。PTTG可能通过激活bFGF蛋白表达促进新生血管生成而促使卵巢上皮性癌发生转移。

OBJECTIVE:To investigate the expression of pituitary tumor-transforming gene (PTTG) in epithelial ovarian cancer and its relations with the microvessel density (MVD) and the expression of basic fibroblast growth factor (bFGF) protein. METHODS: The expression of PTTG mRNA was assessed by the reverse transcription polymerase chain reaction in 42 cases of epithelial ovarian cancer; the expression of PTTG protein and bFGF protein were studied by the immunohistochemical method. Microvessels were highlighted by staining endothelial cells with CD_ 34 antigen, and MVDs were counted. RESULTS: Expression of PTTG was detected in a significantly greater proportion in epithelial ovarian cancer than benign tumors and normal tissue. The expression of PTTG was related to the surgical-pathological stage and lymph node metastasis, P<0.05. There was no relationship between PTTG expression and age, pathological type and histological grade, P>0.05. Expression of bFGF protein and MVD was positively correlated with expression of PTTG, P<0.05. CONCLUSIONS: Increased PTTG may play important roles in carcinogenesis and development of epithelial ovarian cancer by promoting the expression of bFGF protein which induces an angiogenesis.