MMP-2与TIMP-2的比值与食管癌侵袭转移的关系

Relationship between ratio of MMP-2 and TIMP-2 and infiltration metastasis of esophageal carcinoma

目的探讨基质金属蛋白酶-2(ma-trix metalloproteinase-2,MMP-2)及基质金属蛋白酶组织抑制剂-2(tissue inhibitor of met-alloproteinase-2,TI MP-2)的表达与食管癌侵袭转移的关系。方法通过免疫组化和流式细胞术(flowcytometry,FCM)检测30例食管癌组织标本和5例正常食管组织标本的MMP-2及TI MP-2基因蛋白的表达情况,采用HPIAS-1000病理图文分析系统,对切片上的组化染色信号进行灰度分析。结果食管癌组织中MMP-2的净灰度值为64.57±24.91,食管正常组织为16.57±7.91;食管癌MMP-2的表达量FI=1.42±0.24,食管正常组织为1.00±0.09;食管癌MMP-2的表达明显高于正常组织,t=2.441,P=0.02。食管癌组织中TI MP-2的净灰度值为45.61±27.82,食管正常组织中为13.89±7.34;食管癌TI MP-2的表达量FI=0.97±0.19,正常组织为1.00±0.15;食管癌TI MP-2的表达与正常组织差异无统计学意义。免疫组化测食管癌MMP-2/TI MP-2值为1.72±0.06,正常组织为0.96±0.43;FCM测食管癌MMP-2/TI MP-2值为1.53±0.04,正常组织为1.02±0.24;食管癌MMP-2/TI MP-2值明显高于正常组织,P<0.05。MMP-2、MMP-2/TI MP-2与浸润深度、淋巴结转移密切相关,P<0.05;但与食管癌的分化程度无关。结论MMP-2/TI MP-2与食管癌侵袭转移密切相关,有助于确定术后治疗方案。

OBJECTIVE:To investigate the role of expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in the invasion and metastasis of esophageal carcinoma. METHODS: Tissues were obtained from 30 patients with esophageal carcinoma and 5 normal esophageal tissues. Expressions of MMP-2 and TIMP-2 were examined by immunohistochemical staining (IHC) and flow cytometry (FCM) technique. The results of IHC were applied to HPIAS-1000 image analysis system and translated to gray values. RESULTS: The grey value of MMP-2 in esophageal carcinoma was 64.57±24.91 and in normal esophageal tissues was 16.57±7.91. Expression of MMP-2 in esophageal carcinoma was 1.42±0.24 by FCM, in normal esophageal tissues was 1.00±0.09 by FCM. Expression of MMP-2 was significantly higher in esophageal carcinoma than that in normal esophageal tissues, t=2.441,P=0.02. The grey value of TIMP-2 in esophageal carcinoma was 45.61±27.82 and in normal esophageal tissues was 13.89±7.34. Expression of TIMP-2 in esophageal carcinoma was 0.97±0.19 by FCM and in normal esophageal tissues was 1.00±0.15 by FCM. No apparent correlation was found between expression of TIMP-2 in esophageal carcinoma that in normal esophageal tissues. MMP-2/TIMP-2 in esophageal carcinoma was 1.72±0.06 by IHC, 0.96±0.43 in normal tissues by IHC. MMP-2/TIMP-2 in esophageal carcinoma was 1.53±0.04 by FCM, MMP-2/TIMP-2 in normal esophageal tissues was 1.02±0.24 by FCM. Significant relationship was found between MMP-2/TIMP-2 in esophageal carcinoma and that in normal esophageal tissues, P<0.05. Expression of MMP-2 and MMP-2/TIMP-2 were significantly correlated with the invasion and lymph node metastasis, P<0.05, and did not correlate with the histological classification. CONCLUSION: MMP-2/TIMP-2 may contribute to the invasive properties of esophageal carcinoma, and be helpful in designing treatment protocols.