中枢神经系统疾病患儿脑脊液兴奋性氨基酸的变化

EXCITATORY AMINO ACIDS IN CERE-BROSPINAL FLUID IN FOUR COMMON DISEASES OF CENTRAL NERVOUS SYS-TEM IN CHILDHOOD

用丹酰化反应聚酰胺薄层层析及荧光法测定１９例化脓性脑膜炎、６例病毒性脑炎、８例癫痛持续状态（ＳＥ）和２１例热性惊厥患儿脑脊液中２２种氨基酸成分。结果表明，与对照组相比，化脓性脑膜炎时１８种氨基酸显著升高，其中谷氢酸、天冬氢酸、丝氨酸及甘氨酸为兴奋性氢基酸。ＳＥ时谷氢酸、天冬氨酸、丝氨酸、赖氨酸及谷氨酞胺显著升高。病毒性脑炎时除谷氨酸不高外，余同ＳＥ。热性惊厥时只有天冬氨酸显著升高。抑制性氨基酸γ－氨基丁酸在各病组均无明显改变。提示在四种常见神经系统疾病时脑内存在兴奋毒性。

oncentrations of 22 amino acid components incerebrospinal fluid (CSF)in children suffering frompurulent meningitis ( PM,n=19), viral encephalitis(VE,n=6),status epilepticus (SE,n=8) and febrileseizures(FS,n=19) were determined by dimethylamino-naphthalene-S-sulfonyl chloride with thin layerchromatography(TLC) and fluorometry. As signifi-cant rise of 18 out of 22 amino acids in CSF in PM wasassumed to be a concequence of leakage of blood-CSFbarrier.But the rise of excitatory amino acids such asglutamate ,aspartate ,glycine and serine in CSF shouldbe regarded a fortiori as the products chiefly of brainitself.Since it is known that there is no barrier dvs-function in VE,SE,and FS,the elevation of excitato-rv amino acids levels mentioned above probably has anintrinsic mechanism in the brain. However, a uniquefinding among all the diseased groups was the elevationof aspartate which is thought of the most sensitive oneto cerebral hypoxia. On the other hand,the inhibitoryamino acid GABA had no significant change in allgroups. These data suggest that a development of exci-totoxicity exists in brain in the 4 common central ner-vous system diseases.