摘要
采用IgH和TcRy基因重排两个标志相结合的方法,通过聚合酶链反应(PCR)检测微小残留病(MRD)。18例急性淋巴细胞白血病(ALL)患儿在终止治疗后有9例检出MRD。受检病例在检测后随访1.5~102个月,结果9例MRD阳性者有3例于3~6个月内复发;而MRD阴性的9例无1例复发,提示治疗终止后仍存在MRD者以后有复发的危险,应进行定期监测;MRD阴性可作为临床停药观察的一个客观指标。该方法检测MRD简便、阳性率较高,适于临床应用。
ith the IgH and TCRγ gene rearrangements asgene markers,we detected minimal residual disease(MRD) by means of polymerase chain reaction(PCR ). Of 18 cases with childhood acute lymphoblas-tic leukernia,9 patients had MRDs after completion oftherapy, All patients had been followed-up for 1.5 to102 months since detection.Three out of the 9 MRD-positive patients relapsed within 3 to 6 months; How-ever,no one among the 9 MRD-negative cases re-lapsed. These results suggested that the patients withMRD after cessation of therapy should be monitoredperiodically for future relapses,and that the “MRD-negative”might be a useful objective indicator for end-ing therapy. The method we used to detect MRD inthis study was simple,convenient and sensitive,hencesuitable for clinical examination.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
1995年第6期352-354,共3页
Chinese Journal of Pediatrics
关键词
白血病
淋巴细胞性
聚合酶链反应
Leukemia,Iymphoblastic,acutePolvmerase chain reaction