婴儿沙眼衣原体肺炎病原学和临床的研究

ISOLATION AND IDENTIFECATION OFCHLAMYDIA TRACHOMATIS IN INFFANTSWITH PNEUMONIA

为证实婴儿肺炎中沙眼衣原体的感染，应用ＨｅＬａ－２２９细胞对４９例肺炎婴儿的鼻咽部分泌物进行沙眼衣原体（ＣＴ）培养分离，以特异的单克隆荧光抗体进行鉴定；同时应用酶联免疫吸附试验检测患儿血清中ＣＴ特异性ＩｇＭ。结果其中９例患儿（最小４天，最大８个月）的标本于荧光显微镜下检测到典型ＣＴ包涵体荧光颗粒、并于相应的血清中检测到高效价ＩｇＭ，检出率为１８．４％（９／４９），其中６个月以下婴儿为２３．５％（８／３４）。９例患儿病初均未采用红霉素治疗，病程迁延最长达４４天，平均２０天。经研究证实，ＣＴ确为婴儿肺炎的重要病原体，故对婴儿肺炎，尤其６个月以下婴儿肺炎，应高度注意ＣＴ感染的可能；早期确诊、及时应用ＣＴ敏感药物，方能取得较好疗效。

e carried out isolation and identification ofChlamydia trachomatis (CT) from nasopharyngealsecretions(NPS) in 49 infants with pneumonia usingHela-229 cells and specific monoclonal fluorescent anti-body (MFA)technique, and assayed serum IgM anti-body to CT of culture positive infants using enzyme-linked immunosorbent assay(ELISA)from April toDecember, 199 3.The results demonstrated that 9cases were CT positive and acquired high titer of IgM. The positive rate of CT pneumonia was 18.4%(9/49),and it was 23.5%(8/34) for infants under 6months of age,Therefore,CT was considered to beone of the important causative agents of pneumonia.