缺氧缺血性脑病新生鼠补钙前后脑细胞、红细胞内外钙的变化

CHANGES OF INTRA- AND EXTRA-CEL-LULAR CALCIUM CONCENTRATIONS IN BRAIN CELLS AND ERYTHROCYTES PRI-OR TO AND POST CALCIUM SUPPLEMEN-TATION IN NEONATAL RAT HYPOXIC IS-CHEMIC ENCEPHALOPATHY MODEL

为了观察一般剂量补钙对缺氧缺血性脑病（ＨＩＥ）脑细胞和红细胞内外钙变化的影响，以探讨ＨＩＥ时补钙是否增加脑细胞钙超载，用ＨＩＥ新生动物模型进行了实验研究，结果表明：（１）缺氧缺血时红细胞、脑细胞及脑组织出现钙积聚；（２）补钙后，血浆总钙浓度明显升高，而脑细胞、脑组织及红细胞钙积聚并未加重；（３）血浆总钙浓度与脑细胞或红细胞胞浆游离钙离子浓度无相关关系。提示：（１）ＨＩＥ时钙内流可能是全身性的；（２）对ＨＩＥ并低钙血症患儿吸氧后一般剂量补钙可能是安全的。

n order to determine whether calcium supplemen-tation during the development of hypoxic ischemicencephalopathy (HIE) would aggravate calcium over-load in brain cells,we examined the effect of supple-mentation of calcium on changes of intra-and extracell ular calcium concentration in neonatal rat HIEmodel.The results showed that(1)there were calci-um overloads in brain cells,brain tissues as well as inthe erythrocytes during the development of HIE;(2)after calcium supplementation ,plasma calcium concen-tration increased significantly,while calcium overloadsin brain cells,brain tissues and erythrocytes were notaggravated; and (3)plasma calcium concentration wasnot correlated with intracellular free calcium concentra-tion either in brain cells or in erythrocytes in anygroup. The results indicate that(1) influx of calciuminto cells occurs not only in brain cells,but also in ery-throcytes and therefore,it may be a systemic processduring the development of HIE;(2)ordinary dose ofcalcium supplementation may be safe to HIE neonatescomplicated with hypocalcemia after reoxygenation.