秸秆固态发酵酒精过程中参数的测定

Study on the Measure of Parameter during Solid Fermentation of Ethanol with Corn Stover

研究发现菌体生物量与麦角固醇含量间存在良好的线性关系,通过测定发酵底物中麦角固醇含量就能推测出菌体的生物量。康氏木霉菌龄对氨基葡糖含量的影响很大,故不宜作为测定菌体生物量的方法。发酵培养基本身含有的麦角固醇含量不能忽略,可通过方程推测菌体生物量;利用干重损失法推测生物量存在一定的缺陷;呼吸强度的变化趋势与菌体的比生长速率曲线具有较好的相关性,说明呼吸强度的变化能在一定程度上反映菌体的生长状况。

The mycelium biomass of Trichoderma koniggi TR6 and Saccharomyces cerevisiae CB2 were found to has a good linear relation with the content of ergosterine. Engosterine in the broth could be used to predict the mycelium biomass. The amount of ergosterine could not be neglected since the culture medium itself contains ergosterine. The traditional method to determine mycelium biomass by weighing dry cell mass loss had its drawbacks. It was found that the changes of breathing intensity showed a strong correlation with the growth rate of mycelium, which could be used to determine the mycelium growth state.