大鼠鼻粘膜降钙素基因相关肽能神经末梢的分布

Distribution of Calcitonin Gene-related Peptidergic Terminals in Rat Nasal Mucosa

用免疫细胞化学技术（ＡＢＣ法）观察大鼠鼻粘膜降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅ－ｒｅ－ｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ能神经末梢的分布。结果表明，ＣＧＲＰ能神经末梢广泛分布于小动脉壁、小静脉壁、腺泡周围及上皮细胞间，其中以小动脉壁密度最大。ＣＧＲＰ能神经末梢的典型形态为多级分枝的串珠样纤维。三叉神经节中也可见ＣＧＲＰ免疫反应阳性神经元胞体。基于上述观察，认为鼻粘膜中ＣＧＲＰ能末梢属于三叉神经节细胞伸向鼻粘膜的外周纤维，它们参与感受鼻粘膜局部刺激，及向中枢性末梢传递神经冲动，并在脑干释放ＣＧＲＰ。ＣＧＲＰ也可能在外周鼻粘膜局部释放并影响粘膜功能。

The distribution of calcitonin gene-related peptidergic(CGRP)terminals in rat nasal mu-cosa was observed with immunocytochemistry technioue(ABC method).The results showed that CGRP-ergic terminals had extensive localizations along the walls of arterioles and venules,around the acini of glands and between the epithelial cells, with the walls of arterioles having highiest densities of such terminals.The typical morphology of CGRP-ergic terminals was one of multistage branched fibers in the form of rosary varicosities. CGRP immunoreactive neurons were also present in trigeminal gan-glion(TG).Based on our observations,it is suggested that CGRP-ergic terminals in rat nasal mucosa are the peripheral fibers originating from the ganglion cells in TG. These fibers may be involved in the perception of local mucosal stimuli and propagation of neural impulses to the central terminals and will then release CGRP in the brain stem,There is also possibility that CGRP may be peripherally released in nasal mucosa and exerts influences on mucosal functions.