鸡白细胞介素2原核表达载体的构建及表达产物的鉴定

Construction of Chicken Interleukin 2 Prokaryotic Vector and Identification of Expression Product

将鸡白细胞介素2(IL-2)的cDNA克隆到原核表达载体pET-30a(+)上,构建原核重组表达质粒pET-IL-2,并在大肠杆菌BL21(DE3)中诱导表达,37℃用IPTG诱导3.5h后,SDS-PAGE检测表明,表达蛋白以包涵体的形式存在。将诱导后的工程菌超声波裂解,离心后的沉淀用PBS洗涤5～6次,得到高纯度的目的蛋白。

Chicken IL-2 cDNA was cloned into prokaryotic expression plasmid pET-30a(+) to construct recombinant plasmid pET-IL-2,which was expressed in E.coli. BL21(DE3). When the BL21(DE3) was induced by IPTG for 3.5h at 37℃,the expressed product was proved to exist in inclusion body by SDS-PAGE. After being purificated with PBS for 5～6 times,the highly pure product was gotten.