系统性红斑狼疮患者血清中抗内耳组织自身抗体的免疫转印分析

Western Blot Analysis of Serum Autoantibodies Against Inner Ear Antigens in Patients with Systemic Lupus Erythematosus

为了解系统性红斑狼疮（ＳＬＥ）患者血清中是否存在抗内耳组织交叉反应性自身抗体，提取制备豚鼠内耳膜迷路抗原，应用免疫转印技术，对１８例尚无听力下降的ＳＬＥ患者进行分析。结果发现：１６例（占８８．９％）患者血清中存在着抗内耳组织交叉反应性自身抗体，而１１例正常对照血清全部为阴性。提示内耳也可能是ＳＬＥ多器官受损的靶器官之一。

The pupose of this study was to identify the cross-reacting autoantibodies against inner ear antigens in patients with svstemic lupus erythematosus(SLE).Inner ear antigens of guinea pigs were prepared.The sera from 18 patients with SLE without hearing loss and 11 normal subjects were analysed by immunoblotting assty. The results showed that 16 patients with SLE had cross-reacting au-toantibodies,while 11 nomial bjects were all negative(P<0.01),these suggested that the inner ear may be one of the targets invoived by SLE.To further investigate the correlation between hearing status of SLE and autoantibodies against inner ear antigens will be of great value to explore the pathogensis of inner ear insults resulted from certain systemic autoimmune diseases.