人喉上皮细胞体外培养和冷冻保存的研究

Cell Cultrue and Freeze-preservation of the Human Laryngeal Epitkelial Cells

以中晚期引产的胎儿喉上皮组织为材料，研究了喉上皮细胞的体外培养和冷冻保存。结果表明原代培养７天后肉眼可见细胞集落，１０～１４天细胞集落汇合成细胞片或细胞膜片。培养的细胞经简易梯度降温法置液氮（—１９６℃）保存，复苏后仍然具有较强的增殖能力，可供继续研究之用。实验初步解决了喉上皮取材，培养条件，冷冻保存等问题，为深入开展这方面的实验研究和建立正常喉上皮细胞株打下了基础。

The laryngeal epithelial cells for cultrue came from healthy fetuses of induced labor of middle and late perid,Cells were cultured under37℃,5%carbon dioxide and saturated humidity. The results showed that in the primary generation was culturcd for 7 days,the aggregens could be seen by the naked eye。In 10～14 days,the cell layers formed and gradually covered the whole bottom of the flasks. The second generation grew faster than the primary one. Twenty flasks of the cuItured cells were freeze-preserved for 14 to 28 days,of which 5 flasks(25%)were resuscitated succesfully, By means of the cell culture,some characteristics of the laryngeal epithelial cells can be studied.