喉癌细胞增殖活性与组织学恶性度及预后关系的研究

The Proliferative Activity of Laryngeal Carclnoma and its Relatlonship to Histological Malignaucy and Proguosis

应用增殖细胞核抗原（ＰＣＮＡ）单克隆抗体ＰＣ１０免疫组化法、ＤＮＡ图像分析法及核分裂像计数法对７０例喉鳞状细胞癌的增殖活性进行检测。结果显示，王种方法的检测结果具有较好的一致性。细胞增殖活性与喉癌的组织学分化程度及患者的五年生存率有密切关系（Ｐ＜０．０１，Ｐ＜０．０１）。结果表明，检测肿瘤细胞增殖活性对于判断喉癌的恶性度及估计患者的预后具有重要的意义。

Cellular proliferation indices such as proliferating cell nuclear antigen(PCNA) ,DNA content index and mitotic figure counts ( MFC )were evaluated in 7 0 cases of laryngeal squamous cell carcinoma. The results showed that there were strong correlations between these indices and histological grading of the carcinoma(P<0. 01,P<0.01, P<0.05).Both PCNA index and MFC were related to the five-year survival rate(P<0. 01).The PCNA Iabelling index was corresponded with both DNA in-dex and MFC(r=0. 61,P<0. 001;r=0.76,P<0. 001)。Our data suggest that cellular proliferation of laryngeal carcinoma can be reliably assessed with three different methods and these indices can be used as the predictor of tumor malignancy and prognosis.