喉癌P_（53）蛋白表达的临床意义

Expression of P_(53) Protein in Laryngeal Carcinoma and Its Clinical Significance

以免疫组化方法，用单克隆抗体ＤＯ－７检测了３８例喉鳞癌和２９例喉部良性病变组织中的突变型Ｐ＿（５３）蛋白的表达情况。结果显示：①６６％（２５／３８）的喉鳞场中检出突变型Ｐ＿（５３）蛋白，喉良性病变突变型Ｐ＿（５３）蛋白的阳性率仅为７％（２／２９）。突变型Ｐ＿（５３）蛋白在喉癌中的高比率表达与喉癌的发生有关。以该蛋白是否表达作为喉癌的诊断和鉴别诊断的辅助依据，具有一定价值。②在部分喉鳞场的癌旁呈不典型增生的上皮中，也可见突变型Ｐ＿（５３）蛋白的表达。癌旁组织中已发生Ｐ＿（５３）基因突变的细胞可能是肿瘤复发的根源或先兆。③突变型Ｐ＿（５３）蛋白阴性的喉癌患者５年生存率比阳性患者高。其表达具有一定预后意义。

Using the monoclonal antibody DO-7, the expressions of P_(53) protein in 38 cases of laryn- geal squmaous cell carcinoma (LSCC),and 29 cases of laryngeal benign neoplasms( LBN)were studied immunohistochemically. The results showed that:①25 of 38(66%)LSCCs, but only 2 of 29(7%)LBNs showed expression of mutant-type P_(53) protein. The high rate of P_(53) protein expression was related to the occurrence of laryngeal carcinoma,and is valuable in diagnosis and differential diagnosis of laryn- geal carcinoma;②in some LSCCs,the mutant-type P_(53) protein was also expressed in dysplastic epitheli- um near the cancer tissues. These epithelial cells near the tumor in which P_(53) gene has been altered,probably are the source or portent of tumor recurrence;③the five year survival rate of mutant-type P_(53) protein negative LSCC patients was higher than that of positive LsCC patients. The expreesLion of mu- tant-type P_(53) protein might be related to LSCC prognosis.