菝葜的抗炎活性成分研究

A Study of the Anti-inflammatory Components of Smilax China

目的研究菝葜的抗炎活性成分及其抗炎作用。方法采用反复硅胶柱层析法对菝葜乙酸乙酯部位的化学成分进行分离纯化,分别采用红外、质谱、磁共振(包括1H-NMR、13C-NMR及2D-NMR)方法鉴定所得化合物的结构。并通过大鼠灌胃给药,考察所得化合物对大鼠棉球肉芽肿的影响。结果共分离鉴定了2种化合物,即白藜芦醇(化合物Ⅰ)和氧化白藜芦醇(化合物Ⅱ)。化合物Ⅰ0.2mg·kg-1·d-1对大鼠棉球肉芽肿有显著的抑制作用(P<0.05)。结论白藜芦醇是菝葜的抗炎活性成分之一。

Objective To study the active anti-inflammatory components of Smilax China and their anti-inflammatory effects in rats. Methods The chemical components of the ethyl acetate portion of Similax China were separated and purified repeatedly with silica gel column chromatography. The structures of the chemical compounds Ⅰ and Ⅱ thus obtained were then authenticated with infrared analysis, mass spectrometry and nuclear magnetic resonance (including ~1H-NMR,~13C-NMR and 2D-NMR),separately. The anti-inflammatory activity of the compounds Ⅰ and Ⅱ was assayed by observing the effect of the compounds on the cotton ball-induced granuloma in rats after these compounds had been administered by gastrogavage. Results The compoundsⅠandⅡ isolated were identified as reverstroal(Ⅰ) and oxyreverstroal(Ⅱ). Reverstroal (Ⅰ) in a dose of 0.2 mg·kg^-1·d^-1 was shown to inhibit the cotton ball-induced granuloma in rats(P<0.05). Conclusion Reverstroal (Ⅰ) was found to be one of the anti-inflammatory components of Smilax China.