近红外漫反射光谱法定量分析注射用二乙酰氨乙酸乙二胺

Quantitative Assay of Ethylenediamine Diaceturate in Its Injection with Near-infrared Diffuse Reflectance Spectroscopy

目的建立近红外漫反射光谱分析技术定量测定注射用二乙酰氨乙酸乙二胺含量的方法。方法采用傅立叶变换近红外光谱仪和固体光纤探头,采用InGaAs检测器,在12000~4000cm-1范围内每份样品扫描64次,求平均值,通过偏最小二乘法建立模型,样品建模采用波段为7482.5~5041.1cm-1。结果定量分析模型对未知样品的预测均方差RMSEP为2.03,预测值与真实值的相关系数R为0.9969,平均回收率为(100.85±0.29)%,方法精密度RSD为0.28%。结论用近红外漫反射光谱分析技术对注射用二乙酰氨乙酸乙二胺进行定量分析,结果准确可靠,方法简便快速,不需预处理,适合大量重复性样品的分析测定。

Objective To set up an method for the quantitative determination of ethylenediamine diaceturate in its injection with near-infrared diffuse reflectance spectroscopy. Methods All spectra were surveyed with a Fourier transform spectrometer equipped with a InGaAs detector and a fibre-optic probe for reflectance measurements. All samples were scanned from 12 000·cm^-1 to 4 000·cm^-1,and each sample spectrum was obtained as an automatic mean of 64 scans. Spectral region7 482.5-5 041.1·cm^-1 was selected to develop mathematical models by the partial least square method. Results The root mean square error of prediction (RMSEP) obtained from the validation of 10 unknown samples was 2.03,the correlation coefficient between the true value and prediction value was 0.996 9. The average rate of recovery was (100.85±0.29)%,and RSD of the method precision, 0.28%. Conclusion The results show that the method is accurate, reliable, simple and convenient for the determination of ethylenediamine diaceturate in its solution. No pretreatment is required. It is suitable for analyzing high batch samples.