NRSE与NRSF及其对神经元特异性基因表达的调控作用

NRSE,NRSF and Their Modulatory Effects on The Expression of Neuronal-specific Genes

神经限制性沉默元件(NRSE)是一段长度为21￣23bp的保守DNA序列,存在于许多神经元特异表达基因的转录调控区中,神经限制性沉默因子(NRSF)能特异性结合到NRSEdsDNA上,并通过其N端和C端阻遏结构域分别连接共阻遏蛋白Sin3A/B和CoREST,Sin3A招募HDAC对组蛋白进行去乙酰基化修饰,CoREST则作为平台蛋白招募特异的“沉默组件”,以此维持基因沉默.最近的研究显示,NRSEdsRNA能在转录水平与NRSF蛋白直接作用,而不是作为siRNA或miRNA在转录后水平启动神经元特异性基因的表达.

Neuron-restrictive silencer element or repressor element-1 (NRSE/RE1), present in the transcriptional regulatory regions of multiple neuronal-specific genes, is a 21 similar to 23 bp conservative DNA sequence. Neuronal restricted silencing factor or RE1-silencing transcription factor (NRSF/REST) can bind to the NRSE, and then the gene repression was mediated in part through the association of its NH2-terminal repression domain with the corepressor mSin3, resulting in the recruitment of histone deacetylase (HDAC) and consequent acetylization, and its COOH-terminal repression domain with the corepressor CoREST, that may serve as a platform protein for assembly of specialized repressor machinery. The recent study show that NRSE dsRNA can trigger gene expression of neuron-specific genes through interaction with protein NRSF at transcriptional level, rather than through siRNA or miRNA at posttranscriptional level.