小鼠血清中的粒系造血活性及其照后改变

Granulopoietic activity in mouse serum and its changes after irradiation

利用体外半固体琼脂培养骨髓粒单系祖细胞的方法测定小鼠血清中粒系造血活性，经８Ｇｙγ射线照射后４天粒系刺激活性趋于降低；照后７～１０天，显示一定程度的升高，为正常血清的１４０％左右：第１４天呈下降趋势。经３，５，７，９Ｇｙ不同剂量照射后，血清中的粒系造血活性随照射剂量的增加而增高．９Ｇｙ照射组最高，照后第７天可为正常的２００％以上，用后１２天时小鼠中的粒系造血刺激活性仍高于正常血清。照后小鼠血清中亦同时存在粒系造血抑制活性，照后１天即可以观察到，照后１０天前比正常小鼠明显。作者对血清中粒系造血活性升高机理及存在抑制活性对放射病时造血损伤修复的影响进行了探讨。

Using the method of colony formation of CFU-GM, granulopoietic activity in mouse serum was estimated. The experimental results showed that normal mouse serum was capable of stimulating the growth of CFU-GM. This stimulant activity elevated at 7-10th days after irradiation with 8 Gy γ-rays, and the maximal level was 140% of normal serum. At the 14th day post exposure, the increased stimulant acivity tended to decrease. The changes of stimulant granulopoietic activity was also seen in mice irradiated with 3,5,7 ,9 Gy γ-rays. The activity rose with the increasing radiation doses and might last to the 12th day after irradiation. Meanwhile, there was increased inhibitory granulopoietic activity in irradiated mouse serum, especially by 10 days post exposure.The significance of the variation of serum stimulant and inhibitory activities after radiation and its importance in the development of hemopoietic injuries caused by radiation are discussed.