同种Beagle狗循环血造血干细胞移植治疗急性放射病的实验研究

Experimental study on therapy of acute radiation sickness with transplantation of allogeneic peripheral blood hemopoietic stem cells.

实验中用Ｂｅａｇｌｅ狗１０只，以０．０２４７～０．０２７８Ｃ·ｋｇ－１·ｍｉｎ－１照射量率，全身照射６．５Ｇｙ，分３组：第１组３只，作为对照，不给任何治疗，照射后全部死亡，平均活存７．５天。第２组４只，移植性别相反的同种血造血干细胞。供受体混合淋巴细胞培养刺激指数小于０．６９，红细胞交叉凝集试验阴性，未在体外去除供体血细胞中的Ｔ淋巴细胞。结果表明，照射后１１，２０和２６天检查性染色体，均为供体核型。４只狗死于严重ＧＶＨＤ，平均活存４ｌ．６天。第３组３只，移植去除Ｔ淋巴细胞的同种血干细胞，２只死亡，１只发生轻度ＧＶＨＤ，长期活存４年以上。

In the study,10 beagles were used. All the dogs were irradiated with 6.5 Gy of γ-rays from a 60Co source(dose rate,95.6-107.9 R/min) and divided into three groups.All the three dogs in the control group died,having survived 7.5 days on the average after irradiation.In the second group,four dogs were transplanted with allogeneic peripheral blood hemopoietic stem cells(PBHSC) without removing T lymphocytes.The results of sex chromosome tests after irradiation and transplantation showed that the cells were of donor type.All the four dogs died of severe graft-versus-host disease(GVHD) and survived 41.6 days on the average after irradiation.In the third group, three dogs received transplantation of allogeneic PBHSC without T lymphocytes.Two of them died,and the third developed mild GVHD and survived over 4 years.