胎儿弓形体感染防治和产前诊断方法的初步研究

A preliminary study on the antenatal diagnosis and prevention of the fetus toxoplasmosis infection

对有异常妊娠史的孕妇９２例，采用聚合酶链反应（ＰＣＲ）技术筛选出血中弓形体ＤＮＡ（ＴＯＸ－ＤＮＡ）阳性者，进而检测其羊水中ＴＯＸ－ＤＮＡ，并辅助检测羊水γ－谷氨酰转移酶（ＧＧＴ）和甲胎蛋白（ＡＦＰ），对胎儿弓形体先天性感染作出产前诊断，结合药物治疗或引产畸形胎儿。结果：从９２份孕妇血标本中发现ＴＯＸ－ＤＮＡ阳性者９例。其中５例羊水检出ＴＯＸ－ＤＮＡ，另４例羊水中未检出ＴＯＸ－ＤＮＡ。对这９例孕妇产后（或引产后）追踪检测的结果与产前诊断符合。该方法能防止弓形体母婴垂直传播和由此引起的先天畸形。

or those pregnant women with an abnormal pregnancy history, the polymerase chain reaction(PCR)technique was adopted to screening the serum toxoplasmosis DNA (TOX-DNA ). Then tests were made for further evidence of TOX-DNA in the amnionic fluids TOX-DNA was examinated in cases with blood TOX-DNA positive。 Gamma glutamyl transpeptidase (GGT)and a-fetoprotein(AFP) in the amnionic fluids were measured for reference, a antenatal diagnosis of the fetal congenital toxoplas-mosis infection could thus be made。 In the present paper, 9 out of 92 blood specimen were TOX-DNA positive,of the 9 cases, 5 had TOX-DNA and 4 had no TOX-DNA in the amnionic fluid tests。 The re-sults of follow-up examination in the 9 cases were in conformity with the ante natal diagnosis.This is an important procedure for diagnosis and provention of maternal-fetal vertical infection of Toxoplasmosis.