摘要
对有异常妊娠史的孕妇92例,采用聚合酶链反应(PCR)技术筛选出血中弓形体DNA(TOX-DNA)阳性者,进而检测其羊水中TOX-DNA,并辅助检测羊水γ-谷氨酰转移酶(GGT)和甲胎蛋白(AFP),对胎儿弓形体先天性感染作出产前诊断,结合药物治疗或引产畸形胎儿。结果:从92份孕妇血标本中发现TOX-DNA阳性者9例。其中5例羊水检出TOX-DNA,另4例羊水中未检出TOX-DNA。对这9例孕妇产后(或引产后)追踪检测的结果与产前诊断符合。该方法能防止弓形体母婴垂直传播和由此引起的先天畸形。
or those pregnant women with an abnormal pregnancy history, the polymerase chain reaction(PCR)technique was adopted to screening the serum toxoplasmosis DNA (TOX-DNA ). Then tests were made for further evidence of TOX-DNA in the amnionic fluids TOX-DNA was examinated in cases with blood TOX-DNA positive。 Gamma glutamyl transpeptidase (GGT)and a-fetoprotein(AFP) in the amnionic fluids were measured for reference, a antenatal diagnosis of the fetal congenital toxoplas-mosis infection could thus be made。 In the present paper, 9 out of 92 blood specimen were TOX-DNA positive,of the 9 cases, 5 had TOX-DNA and 4 had no TOX-DNA in the amnionic fluid tests。 The re-sults of follow-up examination in the 9 cases were in conformity with the ante natal diagnosis.This is an important procedure for diagnosis and provention of maternal-fetal vertical infection of Toxoplasmosis.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1995年第4期200-202,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
弓形体病
聚合酶链反应
产前诊断
防治
Toxoplasmosis Polymerase Chain reaition Antenatal diagnosis