卵巢癌细胞c－erbB2基因表达与细胞免疫的关系及基因的反义调控

The study of relationship between c-erbB2 expresslon and cytoimmunology in ovarian carcinoma and its antisense regulation

利用卵巢癌细胞株３ＡＯ和ＡＯ，采用Ｓｏｕｔｈｅｒｎ杂交、流式细胞分析和乳酸脱氢酶释放法，在体外进行卵巢癌细胞ｃ－ｅｒｂＢ２表达与细胞免疫的关系及ｃ－ｅｒｂＢ２反义脱氧寡核苷酸的反义调控（ｒｅｇｕｌａｔｉｏｎｏｆｇｅｎｅｅｘｐｒｅｓｓｉｏ）研究。结果：ｃ－ｅｒｂＢ２高表达可抑制肿瘤坏死因子和淋巴因子激活杀伤细胞的杀伤活性，对以过氧化氢为代表的非特异性杀伤活性无明显影响；ｃ－ｅｒｂＢ２反义脱氧寡核苷酸可特异性降低ｃ－ｅｒｂＢ２的高表达，改善肿瘤坏死因子和淋巴因子激活杀伤细胞的杀伤活性。提示：ｃ－ｅｒｂＢ２反义脱氧寡核苷酸富含ｃ－ｅｒｂＢ２高表达，对卵巢癌的治疗有一定作用。

he relationship between c-erbB2 expression and cytoimmunology in ovarian carcinoma cell lines was studied;the synthesized and modified c-erbB2 antisense oligodeoxynucleotide was tested for its abili-ty to reduce over-expression of c-erbB2. The resuIts showed that over-expression of c-erbB2 in ovarian carcinoma could induce resistance to tumor necrosis factor(TNF) and lymphokine activated killer(LAK)cells but had no effect on H_2O_2;c-erbB2 antisense oligodeoxynucleotide could specifically reduce the over-expression of c-erbB2 and increase the sensitivity of ovarian cancer targets to lysis by TNF and LAK cells.