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北京荷斯坦母牛7个微卫星基因座遗传多态性研究 被引量：5

Genetic Polymorphisms of Seven Microsatellite Loci in Beijing Holstein Cows
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摘要 利用7个微卫星基因座BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21对240头北京荷斯坦母牛进行遗传检测,用非变性(中性)聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物。结果表明BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21的等位基因数/多态信息含量/有效等位基因数/杂合度分别为4/0.3869/1.7693/0.4348、5/0.5923/2.9121/0.6566、8/0.7114/3.9012/0.7437、6/0.5921/2.8244/0.6459、6/0.7122/3.9989/0.7499、6/0.7144/4.0124/0.7508和7/0.6154/2.9805/0.6645。可见BM4505的遗传变异最大,BM1818的遗传变异最小。 The polymorphisms of seven microsatellite loci BM1818, BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were detected in 240 Beijing Holstein cows.The PCR amplified products of microsatellites were detected by non-denatured (neutral) polyacrylamide gel electrophoresis. The number of alleles/polymorphic information content/ effective numbers of alleles/heterozygosity of BM1818,BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were 4/0.3869/1.7693/0.4348, 5/0.5923/2.9121/0.6566, 8/0.7114/3.9012/0.7437, 6/0.5921/2.8244/0.6459, 6/0.7122/3.9989/0.7499, 6/0.7144/4.0124/0.7508 and 7/0.6154/2.9805/0.6645, respectively. The results revealed the greatest genetic variation in BM4505 and the lowest in BM1818.
作者 周国利 储明星 金海国 石万海 曹福存 朱颜
机构地区 聊城大学生物系 中国农业科学院畜牧研究所 延边大学农学院动物科学系 北京奶牛中心
出处 《安徽农业大学学报》 CAS CSCD 北大核心 2005年第3期293-297,共5页 Journal of Anhui Agricultural University
基金 北京市自然科学基金项目(6002014)资助。
关键词 北京荷斯坦母牛 微卫星基因座 遗传多态性 <Keyword>Beijing Holstein cows microsatellite loci genetic polymorphisms
分类号 S823.912 [农业科学—畜牧学]
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参考文献12

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安徽农业大学学报
2005年 第3期

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