摘要
利用7个微卫星基因座BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21对240头北京荷斯坦母牛进行遗传检测,用非变性(中性)聚丙烯酰胺凝胶电泳检测微卫星的PCR扩增产物。结果表明BM1818、BM1258、BM1443、BM1905、BM302、BM4505和CYP21的等位基因数/多态信息含量/有效等位基因数/杂合度分别为4/0.3869/1.7693/0.4348、5/0.5923/2.9121/0.6566、8/0.7114/3.9012/0.7437、6/0.5921/2.8244/0.6459、6/0.7122/3.9989/0.7499、6/0.7144/4.0124/0.7508和7/0.6154/2.9805/0.6645。可见BM4505的遗传变异最大,BM1818的遗传变异最小。
The polymorphisms of seven microsatellite loci BM1818, BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were detected in 240 Beijing Holstein cows.The PCR amplified products of microsatellites were detected by non-denatured (neutral) polyacrylamide gel electrophoresis. The number of alleles/polymorphic information content/ effective numbers of alleles/heterozygosity of BM1818,BM1258, BM1443, BM1905, BM302, BM4505 and CYP21 were 4/0.3869/1.7693/0.4348, 5/0.5923/2.9121/0.6566, 8/0.7114/3.9012/0.7437, 6/0.5921/2.8244/0.6459, 6/0.7122/3.9989/0.7499, 6/0.7144/4.0124/0.7508 and 7/0.6154/2.9805/0.6645, respectively. The results revealed the greatest genetic variation in BM4505 and the lowest in BM1818.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2005年第3期293-297,共5页
Journal of Anhui Agricultural University
基金
北京市自然科学基金项目(6002014)资助。
关键词
北京荷斯坦母牛
微卫星基因座
遗传多态性
<Keyword>Beijing Holstein cows
microsatellite loci
genetic polymorphisms