单克隆抗体L_4B_4在荷人膀胱移行细胞癌裸鼠中的放射免疫显像

RADIOIMMUNOIMAGING IN HUMAN TRANSITIONAL CELL CARCINOMA XENOGRAFTED NUDE MICE WITH MONOCLONAL ANTIBODY L_4B_4

目的：研制与膀胱移行上皮癌（ＴＣＣ）特异结合的单克隆抗体Ｌ４Ｂ４。方法：用免疫组化法评价Ｌ４Ｂ４，并对荷人ＴＣＣ裸鼠行放射免疫显像（ＲＩＩ）。结果：免疫组化研究显示：Ｌ４Ｂ４对ＴＣＣ具有很高的特异性（２３／２４），而很少与其他恶性肿瘤（２／２４）及良性肿瘤（０／７）相结合。荷瘤裸鼠ＲＩＩ研究显示：注入１２５Ｉ－Ｌ４Ｂ４后第３天及第５天可清晰显示肿瘤影像，第５天的Ｔ／ＮＴ＞４。结论：Ｌ４Ｂ４可在膀胱癌的ＲＩＩ研究中发挥作用，值得进一步研究。

PURPOSE The monoclonal antibody L4B4 against transitional cell carcinoma(TCC)was prepared and radioimmunoimaging(RII)was studied in nude mice bearing human TCC using L4B4.METHODS L4B4 was identified in vitro by immunohistochemistry.Radioimmunoimaging was performed in human transitional cell carcinoma xenografted nude mice.RESULTS Immunohistochemistry study showed that L4B4 had high specificity for TCC(23/24),compared to that for other malignant tumors(2/24)and benign tumors (0/7).RII study showed that xenografted tumor was demonstrated clearly on the 3rd and 5th day after injection of 125I labeled L4B4.The T/NT was greater than 4 on the sib day.CONCLUSION Our results indicated that L4B4 might be useful in the study of TCC and is worth further investigation.