摘要
目的:研制与膀胱移行上皮癌(TCC)特异结合的单克隆抗体L4B4。方法:用免疫组化法评价L4B4,并对荷人TCC裸鼠行放射免疫显像(RII)。结果:免疫组化研究显示:L4B4对TCC具有很高的特异性(23/24),而很少与其他恶性肿瘤(2/24)及良性肿瘤(0/7)相结合。荷瘤裸鼠RII研究显示:注入125I-L4B4后第3天及第5天可清晰显示肿瘤影像,第5天的T/NT>4。结论:L4B4可在膀胱癌的RII研究中发挥作用,值得进一步研究。
PURPOSE The monoclonal antibody L4B4 against transitional cell carcinoma(TCC)was prepared and radioimmunoimaging(RII)was studied in nude mice bearing human TCC using L4B4.METHODS L4B4 was identified in vitro by immunohistochemistry.Radioimmunoimaging was performed in human transitional cell carcinoma xenografted nude mice.RESULTS Immunohistochemistry study showed that L4B4 had high specificity for TCC(23/24),compared to that for other malignant tumors(2/24)and benign tumors (0/7).RII study showed that xenografted tumor was demonstrated clearly on the 3rd and 5th day after injection of 125I labeled L4B4.The T/NT was greater than 4 on the sib day.CONCLUSION Our results indicated that L4B4 might be useful in the study of TCC and is worth further investigation.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
1995年第4期245-246,共2页
Chinese Journal of Nuclear Medicine