接触高温、CO、粉尘作业工人外周血淋巴细胞DNA的损伤

ABSTRACTS OF ORIGINAL ARTICLES IN ENGL ISH STUDY ON DNA DAMAGE OF LYMPHOCYTES IN WORKERS EXPOSED TO HEAT,CARBON MONOXIDE AND DUST

应用单细胞凝胶电泳检测技术（ＳｉｎｇｌｅＣｅｌｌＧｅｌＡｓｓａｙ，ＳＣＧ）对某钢厂不同生产车间工人外周血淋巴细胞单链ＤＮＡ损伤进行检测。结果表明，ＣＯ组、ＣＯ＋高温组工人的ＤＮＡ损伤发生率高于高温组、粉尘组和对照组４～６倍，其ＤＮＡ损伤的程度亦较严重（以接触ＣＯ者为最严重）（Ｐ＜０．０１），而高温组、粉尘组和对照组之间差异无显著性（Ｐ＞０．０５），提示外周血淋巴细胞单链ＤＮＡ损伤率有可能用作职业有害因素对健康影响的早期监测指标。

he single cell gell( SCG )assay (namely,comet assay)is a sensitive electrophoretic technique for detecting the presence of DNA single strand breaks and alkali-labile damage in individual cell.The technique was used to evaluate the levels of DNA darmage in peripheral blood lymphocytes of the workers exposed to heat,CO,CO+heat and dust.The subjects in control group were the personnel with no occupational exposure history.The percentage of DNA damage in CO,CO+heat were 4～6 times higher than those in heat,dust and control.The darnaged levels were more severe too. The previous data of the worker’s health indicated that cancer incidence of the CO group and CO+heat group was 7 times higher than that of the control.t is suggested that the rate of DNA damage in lymphocytes may be an early health monitoririg index for workers in some occupational hazards.