实验性铍病支气管肺泡灌洗液中细胞免疫学观察

CELLULAR IMMUNITY IN BALF OF EXPERIMENTAL BERYLLIUM LUNG DISEA5E IN GUINEA PIGS

以氧化被气道染毒豚鼠，观察链病豚鼠支气管肺泡灌洗液（ＢＡＬＦ）细胞成分、淋巴细胞对铍盐的增殖反应及肺泡巨噬细胞（ＡＭ）产生白细胞介素－１（ＩＬ－１）的变化。发现被染毒早期ＢＡＬＦ中多形核粒细胞显著增加，后期则淋巴细胞增加明显。与外周血铍淋转比较，ＢＡＩＦ淋巴细胞对铍盐刺激的增殖反应更为敏感。实验说明铍染毒后期豚鼠肺部病变与细胞免疫异常有关，不但肺部发生免疫性肉芽肿病，同时也存在淋巴细胞性过敏性肺炎。氧化铍能刺激铍病豚鼠及正常豚鼠的ＡＭ产生ＩＬ－１，提示氧化铍对ＡＭ可能具有激活作用。

n experimental model for beryllium disease was produced in guinea pigs by endotracheal injection of beryllium oxide(BeO),the changes of cellular immunity in bronchoalveolar lavage fluid(BALF)were observed.The results showed that BALF cells signif-icantly increased in experimental animals,polymorphonucleocytes increased in early stage,and lymphocytes increased in late stageafter BeO administration ,the proliferative response of BALF lymphocytes to beryllium was stronger than the lymphocytes in pe-ripheral blood.These results indicated that BeO induced lung disease in guinea pigs was close related to cell-mediated immunolog-cal reaction. This disease is not only a pulmonary immunological granulorna,but also a lymphocyte-mediated hypersensitive pneu-monitis.BeO can stimnulate the alveolar macrophage(AM)both from BeO-treated and non-treated animals to produce IL-l,suggest-ing that BeO may activate the AM and enhance their activities.