摘要
目的:建立金银花药材HPLC指纹图谱。方法:用梯度洗脱法,对金银花药材进行HPLC测定,流动相为1醋酸溶液甲醇线性梯度洗脱,检测波长为254nm。结果:运用梯度洗脱能很好分离金银花的各类成分;经方法学考察,HPLC指纹图谱具有良好的重现性:10批样品中11个特征指纹峰有专属性。结论:本研究建立的金银花药材HPLC指纹图谱分析法可用于金银花药材质量的综合评价。
AIM To establish the HPLC-FPC of Flos Lonicerae . from different growing areas. METHODS The gradient was adopted elution with methanol and 1 % acetic acid, at detection wavelength was at 254 nm. RESULTS The constitutents of Flos Lonicerae were well separated on HPLC, and the HPLC-FPC of 10 batchs of samples was established. The results showed that this method had a good repeatability, and the 11 common peak’s had an exclusive properties. CONCLUSION The method developed can evaluate the quality of Flos Lonicerae conveniently.
出处
《中成药》
CAS
CSCD
北大核心
2005年第7期751-754,共4页
Chinese Traditional Patent Medicine
关键词
金银花
指纹图谱
高效液相色谱
Flos Lonicerae
fingerprint chromatographic
HPLC
gradient elution
