大叶紫薇叶和果实中三萜酸的HPLC/UV/MS指纹图谱比较

Comparison of fingerprints of triterpene acid in the leaves and the seeds of Lagerstroemia specious L by HPLC/UV/MS

目的:采用HPLC/UV/MS对大叶紫薇叶和果实中三萜酸类成分指纹图谱进行了比较。方法:采用LunaC18柱(2.6mm×250mm,5μ);乙腈与0.1甲酸为流动相进行梯度洗脱;流速为1.0mL·min-1。结果:大叶紫薇叶和果实中都含有2α羟基熊果酸和2α羟基齐墩果酸,大叶紫薇叶中含有熊果酸,而果实中含有齐墩果酸。结论:本方法可用于大叶紫薇叶和果实的质量控制。

AIM To develop a comparative method for studying on the HPLC/UV/MS fingerprints of triterpene acid in the leaves and the seeds of Lagerstroemia specious L. METHODS Separation was performed on LunaC_ 18 (2.6 mm×250 mm, 5 μ)analytical column, The mobile phase was composed of acetonitrile and 0.1 % formic acid as gradient eluent. The flow rate was 1.0 ml·min -1 . RESULTS Both of corosolic acid and maslinic acid existed in the leaves and the seeds of Lagerstroemia specious L. Ursolic acid existed in the leaves and the oleanolic acid contained in the seeds of Lagerstroemia speciouswere L. CONCLUSION The method can be used for quality control of the leaves and the seeds of Lagerstroemia specious L.