灵芝三萜对小鼠脾脏树突状细胞增殖的影响

Effect of Ganoderma Triterpene on Proliferation of Dendritic Cells from Mouse Spleen

目的:观察灵芝三萜(Ganoderma Triterpene,GT)对小鼠脾脏树突状细胞(Dendritic cells,DC)增殖的影响.方法:采用MTT法,以细胞因子(GM-CSF+IL-4)作比较,观察不同浓度GT以及细胞因子+不同浓度的GT对小鼠脾脏DC的增殖作用.结果:GT在实验浓度范围内(40～200μg/ml)均可显著刺激小鼠脾脏DC增殖,但其刺激强度均明显低于细胞因子组;GT+细胞因子,与对照组相比均有显著的促增殖作用,且明显高于细胞因子组.结论:GT不仅能直接促进小鼠脾脏DC的增殖,而且与细胞因子有显著的协同作用.

Objective: To investigate the effect of Ganoderma Triterpene(GT)on proliferation of Dendritic cells (DC) from mouse spleen. Methods: Compared with the cytokine(GM-CSF+IL-4),we investigated the effect of GT of different concentrations and cytokine+GT of different concentrations on proliferation of DC from mouse spleen by MTT. Results: GT(40～200 μg/ml)could stimulate the proliferation of DC significantly, but the stimulations weren't obvious compared with the cytokine. GT+cytokine, compared with the negative control, had obviously promoting effect and was better than the cytokine. The results above indicated GT could not only stimulate the proliferation of DC but also significantly coorderate with the cytokine. Conclusions: GT may play the role by its growth factor-like function, and by cooperating with the cytokine in the regulation of DC.