塔斯品碱对大鼠皮肤创伤和对成纤维细胞增殖及分泌的影响

Enhancement of Wound Healing by Taspine and Its Effect on Fibroblast

目的:观察塔斯品碱(TA)对大鼠皮肤创伤愈合的作用及对成纤维细胞增殖和分泌生长因子的影响,探讨其促愈合作用机制.方法:建立大鼠皮肤损伤模型,采用组织学的方法评价愈合质量;体外培养L929成纤维细胞,MTT法观察TA对成纤维细胞增殖的影响,ELISA法测定TA对成纤维细胞分泌转化生长因子-β1(TGF-β1)及表皮生长因子(EGF)的影响.结果:TA 3.0和1.5 mg/ml溶液外涂对实验性大鼠皮肤损伤有加速修复作用;体外实验表明,0.05～0.4μg/ml TA可明显增加成纤维细胞对TGF-β1和EGF的分泌,其作用呈剂量依赖性,而对成纤维细胞的增殖无明显影响.结论:TA具有促伤口愈合作用,其作用可能与促进成纤维细胞分泌TGF-β1和EGF有关.

Objective: To study the effect of taspine on enhancement of skin wound healing and its effect on fibroblast proliferation and autocrine. Methods: The plerosis effect of taspine on experimental skin wound was observed in vivo. Different concentrations of taspine were added in vitro and MTT technique was applied to observe its effect on fibroblast proliferation, the levels of transforming growth factor-β_1(TGF-β_1) and epidermal growth factor(EGF) were determined by ELISA. Results: In vivo, exo-applied taspine 300 μg and 150 μg accelerated the recovery of skin wound. In vitro, 0.05～0.4 μg/ml taspine could increase autocrine of TGF-β_1 and EGF by fibroblast, but it showed no effect on L_ 929 fibroblast proliferation. Conclusions: Taspine enhances wound healing by increasing the autocrine of TGF-β_1 and EGF by fibroblast.