转化生长因子－β_1对两个移行细胞癌系的不同调节作用

The differential regulation of TGF-β_1 on two transitional carcinoma cell lines

转化生长因子β１（ＴＧＦ－β１）对两种不同恶性表型的膀胱移行上皮癌具有不同的调节作用。ＤＮＡ合成抑制试验表明，５ｎｇ／ｍｌＴＧＦ－β１可控制分化较好的６４７Ｖ细胞系［３Ｈ］－Ｔｈｙｍｉｄｉｎｅ的结合达６０％；但在同样浓度下不能抑制反而刺激具有转移能力的２５３Ｊ细胞系的ＤＮＡ合成。Ｎｏｒｔｈｅｒｎ杂交分析显示，ＦＮ与ＰＡⅠ－１的表达对６４７Ｖ无明显影响。结果揭示ＴＧＦ－β１对有或无转移能力的肿瘤细胞的不同调节作用可能影响肿瘤的复发与转移。

The effects of TGF-β1 on two transitional carcinorna cell lines were investigated.In a growth inhibition assay determined by 3H-thymidine incorporation, 647V cell line was about 40￣60% inhibited by TGF-β1( 5ng/ml), whereas,253J cell line was not responsive to the inhibitory effect of TGF-β1. By using northern analysis,we assayed gene response of both cell lines to TGF-β1 in term of FN and PAI-1 expression. Both of FN and PAI-1 genes were upregulated in 253J cell line.but slight changes were found in 647V. Thus 253J which was resistant to the growth inhibitory effect of TGF-B1 could response to TGF-β1 by extracellular matrix expression. However,647V which had slight changes of FN and PAI-1 expression was growth inhibited by TGF-B1.Our results suggested that different response to TGF-B1 might be an important distinction between metastatic and nonmetastatic tumor cells and may play an especially important role in tumor recurrence and metastasis.