直接原位聚合酶链反应检测尖锐湿疣中的人乳头瘤病毒

Detection of HPV DNA in Condylomata Acuminata by Direct in Situ PCR

评价用直接原位聚合酶链反应（ＰＣＲ）技术辅助诊断尖锐湿疣（ＣＡ）的价值及观察人乳头瘤病毒（ＨＰＶ）在ＣＡ中的组织学分布模式。２５例ＣＡ中，１０例具典型组织学特征的ＣＡ均有ＨＰＶ分布，主要位于表皮浅层，另 １１例缺乏典型空泡细胞的 ＣＡ在颗粒层或棘细胞全层测得 ＨＰＶ。直接原位 ＰＣＲ通过标记生物素的寡核苷酸在扩增时直接掺入结合，再用免疫组织化学测定，能在石蜡切片上将病毒检测结果与组织学变化直接相比，有助于病理学家对可疑损害作ＨＰＶ感染的分析。

The purpose of this study is to ascertain the significance of diagnosis of human papillomavirus (HPV) infection and the histological pattern of distriution of HPV in condylomata acuminata(CA) by direct in situ PCR (ISPCR) technique. of the 25 patients with BA, all 10 cases with typical histopathological features of CA gave a positive reaction in tile upper layers of the epithelium. HPV was also detected in the granular ladyer and whole prickle cell layer of 11 cases of CA with no typical koilocytes. In the direct ISPCR oligonucleotides labelled with bio tin were incorporated in situ during amplification, then immunohistochemical method was used for detection. In this technique the viral existence in paraffin sections can be directly compared with the histologic changes, and can help pathologist to analyze the possibility that HPV is present in the suspected lesions.