以嗜碱性粒细胞脱颗粒实验研究在真菌过敏性哮喘患者中的应用

Application of human basophil degranulation test(HBDT)in fungal allergic asthmatic patients.

从嗜碱粒细胞水平探讨研究真菌过敏原的方法学，并且应用于过敏性哮喘的研究。实验显示：在ｐＨ７．６、３７℃、真菌抗原浓度１０￣（－１）ｍｇ／ｍｌ的条件下，应用人嗜碱粒细胞脱颗粒实验（ＨＢＤＴ）研究真菌过敏原的方法具有很高的特异性（ｔ＝１６．８，Ｐ＜０．００１）和重复性（ＣＶ：８．７％）。应用ＨＢＤＴ对真菌过敏性哮喘进行研究，发现ＨＢＤＴ与皮肤试验的结果具有很高的符合率（９１．７％）。过敏性哮喘的脱颗粒指数（ＤＩ）显著高于正常人，并且脱敏治疗１年能显著降低其Ｄ１。提示：ＨＢＤＴ是一种简便、准确、可靠的体外研究过敏原的方法，反映了嗜碱粒细胞的功能状况。

he authors recommand the methdology of HBDTfor testing fungal allergens,pH 7.6,37 ℃ and 10mg/ml are the most suitable experimental conditions.The positive rate of HBDT in allergic asthmatic patientsis 91.7%,and correlated very well with the results ofskin tests. The degranulation index(DI)in allergicasthma is significantly higher than that in normals(P<0.001).The DI after one year's desensitization treat-ment with fungal allergens is markedly lowered than be-fore(P<0.001).The results suggest that HBDT is asimple,reliable and precise method to investigate aller-gens in vitro and also reflects the function of basophils inasthma.