肺癌nm23基因产物／核苷二磷酸激酶表达的研究

Immunohistochemical analysis of nm23 gene product/NDPK expression in human lung carcinomas

为了解ｎｍ２３基因在肺癌发生和转移过程中的作用，应用免疫组织化学方法观察了ｎｍ２３基因产物／核苷二磷酸激酶（ｎｍ２３／ＮＤＰＫ）在肺癌的表达情况，结果ｎｍ２３／ＮＤＰＫ的总阳性率为７６％，其中鳞癌７８％，腺癌７２％，明显高于癌旁正常组织，阳性部位在细胞浆。ｎｍ２３／ＮＤＰＫ在腺癌的表达与各种临床病理因素无关；在鳞癌的表达随着肿瘤的增大及淋巴结转移增强，Ⅲ～Ⅳ期癌高于Ⅰ～Ⅱ期癌。结果表明在肺癌ｎｍ２３基因并不具有转移抑制基因的功能，而与肿瘤的发生和进展有关。

t was proposed that nm2 3 gene may function as asuppressor gene for tumor metastasis, Using an anti-body to NDPK, levels of NDPK/nm23 expression inlung carcinomas were examined immunohistochemically,45 of 58 squamous carcinomas and 26 of 36 adenocarci-nomas showed strong imm u noreact ivit y for N DPK/nm23 in most of cancer cells within tumor tissues. Onsome normal bronchial epithelium and serous glands,there also show weak expression. No relationship wasfound between NDPK/nm2 3 expression and clinico-pathological parameters including tumor grade, size,ndal involvement and stage but the increases inNDPK/nm2 3 expression were stronger in advancedstages of squamous cell carcinomas.In conalusion,ourresults indicate that the increased NDPK /nm23 expres-sion in the analysed carci nomas is not consistent withthe proposed metastasis-suppressor function,but thenm23 gene may be implicated in the mechanism of car-cinogenic process and tumor progression.