转化生长因子－β对人胚肺成纤维细胞胶原表达的影响

The effect of transforming growtk factor-β on collagenexpression hy human embryonic fibroblasts.

转化生长因子－β（ＴＧＦ－β）是一族多功能的细胞因子，具有调节细胞外基质代谢的功能，在肺间质纤维化的形成中具有重要作用。胚原是细胞外基质的主要成分。胶原积聚是肺间质纤维化的一个重要特征。为了进一步探讨肺间质纤维化形成的机制，在体外观察了ＴＧＦ－β对人胚胎肺成纤维细胞胶原蛋白合成及胶原Ｉ、胶原ＩＩｌｍＲＮＡ表达的影响。结果发现：ＴＧＦ－β能刺激胶原蛋白的合成，胶原Ｉ、胶原ＩＩＩｍＲＮＡ的表达。而对成纤维细胞的增殖影响不明显。因此认为ＴＧＦ－β可能与肺间质纤维化时胶原积聚有关。ＴＧＦ－β胶原合成的调节是通过ｍＲＮＡ水平。

ransforming growth factor-β (TGFS) is a cy-iokine with diverse biological activity. It can regulatethe metabolic function of extracellular matrix (ECM)and play an important role in the development of pul-monary fibrosis. Collagen are major ECM componentsthat are responsible for normal lung structure and func-tion. Collagen accumulation is a major feature of pul-monary fibrosis. In order to study the mechanism ofpulmonary fibrosis, we examined the effects of TGF-βon the production .mRNA expresstion of type 1 collagenand type Ⅳ collagen by cultures of human embryoniclung fibroblasts. The resulis indicated that TGF-β caninduce collagen forrnation and type Ⅰcollagen, type Ⅳcollagen mRNA exprcssion without affecting cell Prolif-eration. We concluded that TGF-βplays a role in colla-gen accumulation of pulmonary fibrosis and the changesin collagen production appear to be mediated on collagenmRNA level.