肺心病患者绿脓杆菌分型耐药质粒DNA分子杂交的临床意义

The clinical significance of Pseudomonas aeruginosa typing and R-plasmid and DNA molecule hybridization in patients with cor pulmonale

对８所医院住院肺心病患者痰培养获绿脓杆菌（ＰＡ）６００株进行分型分析，结果Ｐ６型占２５．６％。不同地区和医院的假单胞菌及ＰＡ型别分布不同，可作为流行病学调查依据。１５０株中的６８株ＰＡ含有质粒，用β－内酰胺酶和氨基苷钝化酶耐药基因探针各６种，与６０株含质粒的ＰＡ菌落杂交，证实从院内感染患者分离到的３株ＰＡ均带有与ＯＸＡ－２、ＡＡＣ（６＇）－１ｂ同源的ＤＮＡ序列并均来自同一个菌株，在传播院内感染时，又获得了新的耐药基因。

00 strains of Pa were typed from the sputa of pa-tients with cor pulmonale in 8 hospitals. P6 type stoodsfirst, accounting for 25.6%,and the types of PA andthe difference in distribution of Pseudomonas from dif- ferent districts and hospitals, might be used as an im- portant parameter in the epidemiological study.68 of150 strains from patients with cor pulmonale containedplasmids. 60 isolates harbOring plasmids colonies werehybridized with 6 β-lactamase and 6 aminoglycosidemodifying enzyme resistance gene probes, The experi- ments demonstrated the 3 strains isolated from patientswith nosocomial infection came from one same strainand got a new resistance gene during spreading of noso-comial infection, and these isolates contained homolo- gous sequence with OXA-2 and AAC( 6’)-1b probes.