水红花子水提物的抗氧化活性

Antioxidative activity of water-soluble extracts from Fructus Polygoni Orientalis

目的研究水红花子水提物的抗氧化活性及其机制。方法用D-半乳糖建立衰老小鼠模型,分别灌胃服用生药量0.8、1.6和3.2g/kg·b.w.的水红花子水提物(n=10),每日1次,共7周。分别用TBA比色法、亚硝酸盐法、DTNB法及Sohal法测定心、肝、肾中丙二醛(MDA)、超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)及脑中脂褐质(LF)含量。结果衰老小鼠心、肝、肾组织中MDA含量升高,SOD及GSH-PX活性降低,脑组织中LF升高。上述3种剂量的EFPO,能不同程度抑制脑组织中LF升高及心、肝、肾组织中MDA升高,其量效关系均呈负相关,相关系数分别为-0.963(相关性检验P<0.01)、-0.981(P<0.01)、-0.966(P<0.05)及-0.978(P<0.01);能不同程度抑制心、肝、肾组织中SOD活力下降,其量效关系均呈正相关,相关系数分别为0.984(P<0.05)、0.990(P<0.01)、0.985(P<0.01);能不同程度抑制心、肝、肾组织中GSH-PX活力下降,其量效关系均呈正相关,相关系数分别为0.984(P<0.01)、0.956(P<0.01)及0.957(P<0.01)。结论水红花子水提物可通过清除·OH,提高SOD及GSH-PX活力而发挥抗氧化活性。

Objective To study the antioxidative activity of the water-soluble extracts from Fructus Polygoni Orientalis.Methods Malondialdehyde and superoxide dismutase were measured by thiobarbituric acid assay,nitrite method,respectively.The activity of glutathione peroxidase was determined by 5,5'-dithionbis (2-nitrobenzoic acid) photometric method.Lipofuscin was determined by Sohal method in the brain of senile model mice induced by D-galactose.The extracts of 0.8,1.6 and 3.2 g·kg-1·d-1 were administered to the mice separately through oral gavage daily for 7 weeks.Results Malondialdehyde content increased,activities of superoxide dismutase and glutathione peroxidase in the heart,liver and kidney decreased.Levels of lipofuscin in the brain of senile model mice induced by D-galactose increased.The three doses of the extracts could inhibit the levels of lipofuscin in the brain,malondialdehyde in the heart,liver and kidney.These results showed that there was a negative dose-effect relationship.The extracts also up-regulated the levels of superoxide dismutase and glutathione peroxidase in the heart,liver and kidney with a positive dose-effect relationship.Conclusion The water-soluble extracts exert its antioxidative activity by eliminating free hydroxyl radicals,increasing activities of superoxide dismutase and glutathione peroxidase.