摘要
克隆枯草芽孢杆菌纤溶酶(Bacillussubtilisfibrinolyticenzyme,BSFE)基因及其前导肽序列。通过农杆菌EHA105介导转化,获得转基因烟草植株。其BSFE的表达水平为叶片42.97±28.59U·g-1FW、茎15.14±10.57U·g-1FW和根25.55±14.71U·g-1FW。其内源BSFE信号肽可在转基因烟草中行使蛋白转运功能,使BSFE具有分泌表达特性。这一系统可用于建立利用植物组织分泌表达外源蛋白的系统模型。
Bacillus subtilis fibrinolytic enzyme (BSFE) gene with its signal peptide and propeptide sequece were cloned.Transgenic tobacco was obtained by Agrobacterium strain EHA105 mediated transformation.The transgenic tobacco could secret target protein from leaf,stem or root.The amount of BSFE expressed from transgenic tobacco reached 42.97±28.59 U·g^(-1) FW in leaf,15.14±10.57 U·g^(-1) FW in stem and 25.55±14.71 U·g^(-1) FW in root respectively.This system could be used as a model for expression and secretion of exogenous proteins by plant tissues.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第7期1383-1388,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
上海市科委重大项目"安舒克栓酶的药效及工艺研究"(985419000-3)
关键词
枯草芽孢杆菌纤溶酶
转基因
烟草
分泌表达
Bacillus subtilis fibrinolytic enzyme (BSFE)
transgene
tabacco
secretion
