摘要
对水母雪莲细胞悬浮培养过程中细胞生长、黄酮积累和底物消耗的动力学过程进行了研究。经15d液体培养可获得最大生物量干重和黄酮产量分别为17.2g·L-1和607.8mg·L-1,通过调控基本培养基种类和有机添加物可提高雪莲细胞的生长和黄酮积累。获得的水母雪莲细胞培养物具有明显的抗氧化活性,其抗氧化活性与雪莲细胞中的黄酮含量相关。
The kinetic processes of cellular growth,flavonoid accumulation and substrate consumption in the suspension culture of Saussurea medusa cells were studied,which showed that the highest dry biomass and flavonoid yield occurred after 15-day culture amounting to 17.2 g·L^(-1) and 607.8 g·L^(-1),respectively,and the adjustment of the medium kinds and the addition of organic additives could increase the growth and flavonoid accumulation of Saussurea medusa cells.In the culture of Saussurea medusa cells,the antioxidant activities appeared very remarkable and related to flavonoid yield in Saussurea medusa cells.
出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第7期1421-1427,共7页
Acta Botanica Boreali-Occidentalia Sinica
关键词
水母雪莲
黄酮
悬浮细胞
抗氧化活性
Saussurea medusa
flavonoid
suspended cells
antioxidant Saussurea medusa Maxim.is a valuable and traditional Chinese herb of the Compositae family.The whole plant can be used in treating rheumatoid arthritis,regulating menstrual function,and inhibiting ageing and the growth of cancer cells.Phytochemical analysis of S.medusa shows that it is a rich source of total flavonoids including jaceosidin,hispidulin,quercelin and various novel compounds that are difficult to obtain from other plants.These flavonoids are the main medicinally active constituents of S.medusa which in particular display effective anti-tumor and anti-inflammation properties and delay senility^([1]).However,clinical demand for this plant and its total flavonoids products are hardly met due to a very limited supply.The wild S.medusa only grows at rocky beaches of 3 900^(5 100 m) above sea level with a special climate and cultivation of this species in a normal climate has been unsuccessful so far.Owing to over-exploitation of the wild plants for commercial purposes and the difficulty of cultivation, S.medusa is now almost extinct in China.It was listed as the second grade national protected wild plant in China.To solve these problems,large-scale cultivation of plant cells would be a promising alternative to produce useful phytochemicals of S.medusa ,such as flavonoids^([2^4]). The objective of the current study was to obtain a systemic understanding of cell growth,flavonoid accumulation and nutriment exhaustion in the established S.medusa cell suspension cultures,and to set a foundation for efficient large-scale cultivation for biosynthesis of flavonoids.Furthermore,the antioxidant activity of S.medusa cell suspension cultures using the DPPH scavenging assay system was firstly evaluated for further medicinal applications.
