生存素基因表达与脑胶质瘤恶性增殖及凋亡关系的研究

Research on relationship of survivin gene expression with malignant proliferation and apoptosis of brain glioma

目的探讨凋亡蛋白抑制因子生存素(Survivin)基因在脑胶质瘤中的表达水平以及在脑胶质瘤恶性增殖和抗细胞凋亡中的作用。方法选择83例脑胶质瘤标本,采用免疫组化链霉卵白素生物素复合物(SABC)方法检测Survivin和增殖细胞核抗原(PCNA)蛋白的表达,分别计算Survivin免疫反应评分(IRS)和增殖指数(PI);采用TUNEL方法检测脑胶质瘤凋亡细胞,计算凋亡指数(AI)。结果脑胶质瘤SurvivinIRS、PI及AI分别为3.8±3.9、(28.4±19.5)%和(1.0±0.8)%,三者均随脑胶质瘤病理级别的增高而升高(P均<0.01)。Survivin阳性组PI明显高于阴性组PI(P<0.01),PI与SurvivinIRS呈显著正相关(r=0.740,P<0.01)。Survivin阳性组与阴性组之间AI差异无统计学意义(P>0.05),但AI与SurvivinIRS呈显著负相关(r=-0.307,P<0.01)。结论Survivin在脑胶质瘤中高表达,并在脑胶质瘤恶性增殖和抗细胞凋亡过程中发挥重要作用。

Objective To investigate the expression level of inhibitor of apoptosis protein survivin gene in human brain glioma and its role in malignant proliferation and antiapoptosis of brain glioma. Methods Eighty-three cases of brain glioma specimen was chosen, protein expression of survivin and proliferating cell nuclear antigen (PCNA) was investigated by immunohistochemistry streptavidin-biotin complex (SABC) method, the immunoreactivity score (IRS) of survivin and the proliferative index (PI) were counted. Apoptotic cells were screened by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) method, and the apoptotic index (AI) of brain glioma was calculated. Results The survivin IRS, PI and AI of brain glioma were 3.8±3.9, (28.4±19.5)% and (1.0±0.8)% respectively, and all of them were elevated with the increase of pathological grade of brain glioma (P<0.01 for all). PI in survivin positive group was significantly higher than that in survivin negative group (P<0.01), and PI was positively correlated with survivin IRS (r=0.740, P<0.01). There was no significant difference between AI in survivin positive group and that in survivin negative group (P>0.05), however, AI was negatively correlated with survivin IRS (r=-0.307, P<0.01). Conclusions Survivin is overexpressed in brain glioma, and which may play important roles in malignant proliferation and antiapoptosis of brain glioma.