胆管癌组织Smad4和转化生长因子β_1及Ⅱ型受体的表达及意义

Expression of Smad4 and transforming growth factor-β_1, transforming growth factor-β receptor Ⅱ in cholangiocarcinoma tissue and its biological siginificance

目的探讨Smad4和转化生长因子β1(TGFβ1)及Ⅱ型受体(TGFβRⅡ)在胆管癌组织中的表达及与胆管癌生物学行为及预后的关系。方法应用免疫组织化学SP及SABC法检测47例胆管癌及癌旁正常胆管组织中的Smad4和TGFβ1、TβRⅡ的表达,并分析比较与胆管癌患者的临床分期、病理分级等的关系。结果47例胆管癌组织中TGFβ1阳性表达36例(76.6%),较癌旁正常胆管组织高,而Smad4和TGFβRⅡ表达减低,分别为14(29.8%)、28(59.6%)例(P<0.05)。TGFβ1表达与胆管癌临床分期及淋巴结转移和肝转移相关(均P<0.05),与组织学分级无关(P>0.05);TGFβRⅡ表达与胆管癌临床分期相关(P<0.05),与组织学分级及淋巴结转移和肝转移无关(均P>0.05);Smad4表达与胆管癌组织学分级、临床分期及是否淋巴结和肝转移相关(均P<0.05)。结论Smad4、TGFβRⅡ和TGFβ1的表达与胆管癌组织学分级、临床分期及是否淋巴结转移、肝转移有一定的相关性。联合检测三者有助于判断胆管癌的恶性程度和预后。

Objective To study the expression of Smad4 and transforming growth factor-β_1(TGFβ_1), transforming growth factor-β receptor Ⅱ(TGFβRⅡ) in cholangiocarcinoma tissue and its relationship with the biological behaviour and prognosis of the disease. Methods The expressions of Smad4, TGFβ_1 and TGFβRⅡ were detected by immunohistochemical technique in 47 specimens of cholangiocarcinoma and the normal bile duct tissue adjacent to the tumor. The expressions of Smad4, TGFβ_1 and TGFβRⅡ were compared with the clinical stages and pathological grades of the patients. Results The expression of TGFβ_1 was positive in 36 cholangiocarcinomas (76.6%),which was higher than that in the normal tissue adjacent to the lesion. The positive expressions of Smad4 and TGFβRⅡ were 14(29.8%) and 28(59.6%) in the carcinoma tissues, respectively(P<0.05). The expression of TGFβ_1 was related to the clinical stage, metastasis of lymph node and liver of the tumor(P<0.05), but not with the histological grade (P>0.05). There was positive correlation between TGFβRⅡ expression and the clinical stage(P<0.05), but no correlation between the TGFβRⅡ expression and histological grade or metastasis of lymph node and liver(P>0.05). The expression of Smad4 was associated with the histological grade, clinical stage and metastasis of lymph node and liver(P<0.05). Conclusions The expressions of Smad4, TGFβ_1 and TGFβRⅡ correlate with the histological grading, clinical staging and metastasis of the lymph node and liver in cholangiocarcinoma. Combined detection of Smad4, TGFβ_1 and TGFβRⅡ may be helpful in the determination of the malignant degree and the prognosis of this disease.