拉坦前列素滴眼液对人眼球筋膜囊成纤维细胞增殖的影响

Effects of latanoprost eye drops on the proliferation of human Tenon′s fibroblasts in tissue culture

目的研究拉坦前列素滴眼液对体外培养的人眼球筋膜囊成纤维细胞的增殖有无促进作用。方法体外培养人眼球筋膜囊成纤维细胞。按1∶10比例逐级稀释拉坦前列素滴眼液(0.005%),使药物浓度依次为5.00、0.50、0.05μg/ml直至5×10-8μg/ml。采用噻唑蓝比色法检测在不同浓度药物作用下的细胞增殖情况,并在光学显微镜下观察细胞的形态变化。结果较高浓度组(5.00μg/ml)拉坦前列素滴眼液对人眼球筋膜囊成纤维细胞存在明显的杀伤作用,细胞几乎全部死亡,吸光度(A)值与对照组相比明显下降(P<0.01);低浓度组(<0.05μg/ml)A值较对照组无明显变化(P>0.05)。比较拉坦前列素滴眼液在不同作用时间下的剂量效应曲线,24、48及72h差异均无统计学意义(P>0.05)。结论拉坦前列素滴眼液不能促进体外培养的人眼球筋膜囊成纤维细胞的增殖,在较高浓度时具有细胞毒性,在较低浓度时则对细胞增殖无明显影响。

Objective To observe whether there is a direct effect of latanoprost eye drops on the proliferation of cultured human Tenon′s capsule fibroblasts (HTCFs) in vitro.Methods Human Tenon′s capsule fibroblasts were cultured in vitro. Latanoprost eye drops was serially diluted in order to make nine concentrations ranging from 5.00 μg/ml, 0.50 μg/ml, 0.05 μg/ml to 5×10 -8 μg/ml. Fibroblasts growth was evaluated with MTT assay. Morphological changes were observed under phase contrast microscope. Results Majority of cultured human Tenon′s capsule fibroblasts was dead at the concentration of Latanoprost (5 μg/ml) , the A values were significantly decreased (P<0.01). There were no significant changes of the A values (P>0.05) in lower concentration (under 0.05 μg/ml) compared with control. there was no statistical difference in proliferation of HTCFs after latanoprost treatment for 24 hours, 48 hours, 72 hours. Conclusions Latanoprost has the toxic effect on the fibroblast at higher concentrations, but there is no influence on the proliferation of HTCFs at the lower concentrations.